Mobile Phase Optimization in the Analysis of Lysozyme on Zenix^® SEC-300

column

Zenix SEC-300, 30 cm x 4.6 mm I.D., 3 μm particles (Z777028)

mobile phase

as indicated on figure

flow rate

0.35 mL/min

temp.

25 °C

detector

UV, 214 nm

injection

5 μL

sample

lysozyme, 1 mg/mL