HPLC Analysis of Vitamin D₂ and D₃ on Ascentis^® Express C18

column

Ascentis Express C18, 15 cm x 2.1 mm I.D., 2.7 μm particles (53825-U)

mobile phase

acetonitrile

flow rate

0.15 mL/min

column temp.

35 °C

detector

UV, 290 nm

injection

10 μL

sample

10 mg/L each (vitamins D2 and D3) in methanol