HPLC Analysis of Proteins on Antibodix^® WCX-NP10, Comparison of WCX Columns

column

Antibodix WCX-NP10, 25 cm x 4.6 mm I.D., 10 μm particles (Z777272)

mobile phase

(A) 10 mM sodium phosphate buffer, pH 6.0; (B) A + 1.0 M NaCl

gradient

0 to 100% B in 42 min

flow rate

1.0 mL/min

column temp.

ambient

detector

UV, 214 nm

injection

5 μL

sample

1 mg/mL each