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HPLC Analysis of Mephobarbital Enantiomers on Astec® Cellulose DMP

HPLC Analysis of Mephobarbital Enantiomers on Astec® Cellulose DMP application for HPLC

Materials

analytical column

Product No.
Description
Pricing

51099AST

Astec® Cellulose DMP Chiral HPLC Column

5 μm particle size, L × I.D. 25 cm × 4.6 mm

mobile phase component

Product No.
Description
Pricing

270490

2-Propanol

99.5%, HPLC grade

standard

Product No.
Description
Pricing

M3887

Mephobarbital

CONDITIONS

column

Astec Cellulose DMP; 25 cm x 4.6 mm I.D., 5 μm particles (51099AST)

mobile phase

[A] heptane; [B] isopropanol; (80:20, A:B)

flow rate

1 mL/min

column temp.

30 °C

detector

UV, 254 nm

injection

15 μL

sample

mephobarbital, 1 g/L in 50:50, heptane:isopropanol

Description

Analysis Note

Mephobarbital enantiomers are resolved by normal phase chromatography.

Other Notes

Mephobarbital (aka methyphenobarbital) is used as a sedative, anxiolytic and anticonvulsant. It is the N-methylated analogue of phenobarbital, and has similiar indications, therapeutic value, and tolerability.

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany

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