HPLC Analysis of FMOC-Arginine Enantiomers on Astec^® CHIROBIOTIC^® R

column

CHIROBIOTIC R, 25 cm x 4.6 mm I.D., 5 μm particles (13024AST)

mobile phase

0.1% ammonium trifluoroacetic acid in methanol

flow rate

1 mL/min

column temp.

23 °C

detector

UV, 260 nm

injection

2 μL

sample

1 mg/mL in methanol