HPLC Analysis of Diperodon Enantiomers on Astec^® Cellulose DMP, LC/MS Conditions

column

Astec Cellulose DMP, 15 cm x 4.6 mm I.D., 5 μm particles (51098AST)

mobile phase

[A] methanol; [B] ammonium formate; (100:0.1, A:B) (Note: 0.1 w/v ammonium formate, 0.1 g/100 mL)

flow rate

0.5 mL/min

column temp.

25 °C

detector

UV, 230 nm

injection

2 μL

sample

2 mg/mL in mobile phase