HPLC Analysis of Chlorpheniramine Enantiomers on Astec^® CYCLOBOND I 2000

column

CYCLOBOND I 2000, 25 cm x 4.6 mm I.D., 5 μm particles (20024AST)

mobile phase

[A] 1% triethylamine acetate, pH 4.1; [B] acetonitrile; (90:10, A:B)

flow rate

1 mL/min

column temp.

23 °C

detector

UV, 254 nm

injection

2 μL

sample

5 mg/mL in methanol