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A tunable refractive index matching medium for live imaging cells, tissues and model organisms.

eLife (2017-07-15)
Tobias Boothe, Lennart Hilbert, Michael Heide, Lea Berninger, Wieland B Huttner, Vasily Zaburdaev, Nadine L Vastenhouw, Eugene W Myers, David N Drechsel, Jochen C Rink
ABSTRACT

In light microscopy, refractive index mismatches between media and sample cause spherical aberrations that often limit penetration depth and resolution. Optical clearing techniques can alleviate these mismatches, but they are so far limited to fixed samples. We present Iodixanol as a non-toxic medium supplement that allows refractive index matching in live specimens and thus substantially improves image quality in live-imaged primary cell cultures, planarians, zebrafish and human cerebral organoids.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Venor GeM Mycoplasma Detection Kit, PCR-based
Sigma-Aldrich
OptiPrep Density Gradient Medium, used for cell and subcellular organelle isolation