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MP0025

Sigma-Aldrich

Venor GeM Mycoplasma Detection Kit, PCR-based

Synonym(s):
PCR mycoplasma detection kit
NACRES:
NA.75

usage

 kit sufficient for 25 tests

Quality Level

packaging

pkg of 1 kit

storage condition

dry at room temperature

technique(s)

PCR: suitable

application(s)

detection
microbiology

storage temp.

2-8°C

Related Categories

General description

The Venor GeM Mycoplasma Detection Kit utilizes the polymerase chain reaction (PCR), which was established as the method of choice for highest sensitivity in the detection of Mycoplasma and Acholeplasma contamination in cell cultures and other cell culture derived biologicals. Detection requires as little as 1–5fg of mycoplasma DNA corresponding to 2–5 mycoplasma per sample volume. The primer set is specific to the highly conserved rRNA operon, or more specifically, the 16S rRNA coding region in the mycoplasma genome. This allows for detection of all Mycoplasma, Acholeplasma and Ureaplasma species tested so far, which are usually encountered as contaminants in cell cultures. Eukaryotic and bacterial DNA are not amplified by this kit.

Application

The PCR-based Venor GeM Mycoplasma Detection Kit employs PCR technology for rapid and reliable detection of mycoplasma DNA in cell cultures and virus stocks.

Components

Does not include Taq Polymerase. Optimized for use with D9307, Taq DNA Polymerase

Storage and Stability

The kit components are stable during shipping at ambient temperature. Upon receipt, store at 2–8 °C. After rehydration of the primer/nucleotide mix, the positive control, and the internal control, store below –20 °C and avoid repeated freezing and thawing. For repeated testing of low sample numbers, primer/nucleotide mix and controls should be aliquoted after rehydration. By following these recommendations, the kit is stable until the expiration date stated on the label.

Legal Information

Venor is a trademark of Minerva Biolabs GmbH

Kit Components Only

Product No.
Description

  • Positive Control

  • Negative Control

  • PCR 10X Reaction Buffer 500 mL/vial

  • Primer/Nucleotide Mix 1 mL/vial

Storage Class Code

12 - Non Combustible Liquids

Certificate of Analysis

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Certificate of Origin

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