MilliporeSigma

Reversible Aptamer-Au Plasmon Rulers for Secreted Single Molecules.

Nano letters (2015-06-04)
Somin Eunice Lee, Qian Chen, Ramray Bhat, Shayne Petkiewicz, Jessica M Smith, Vivian E Ferry, Ana Luisa Correia, A Paul Alivisatos, Mina J Bissell
ABSTRACT

Plasmon rulers, consisting of pairs of gold nanoparticles, allow single-molecule analysis without photobleaching or blinking; however, current plasmon rulers are irreversible, restricting detection to only single events. Here, we present a reversible plasmon ruler, comprised of coupled gold nanoparticles linked by a single aptamer, capable of binding individual secreted molecules with high specificity. We show that the binding of target secreted molecules to the reversible plasmon ruler is characterized by single-molecule sensitivity, high specificity, and reversibility. Such reversible plasmon rulers should enable dynamic and adaptive live-cell measurement of secreted single molecules in their local microenvironment.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Triton X-100, laboratory grade
Sigma-Aldrich
DAPI, for nucleic acid staining
Sigma-Aldrich
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Sigma-Aldrich
Bovine Serum Albumin, heat shock fraction, suitable for RIA, pH 5.2, ≥96%
Sigma-Aldrich
1,1,2,2-Tetrabromoethane, 98%