Damascena and centifolia roses are cultivated worldwide for their petal extracts that contain key odorant ingredients of perfumes. The analytical identification and quantification of pesticides in rose petals have never been described in the literature. Here, we report on a newly developed method using dispersive solid-phase extraction (d-SPE) cleanup followed by gas chromatography-tandem mass spectrometry for the quantitative determination of multi-residue pesticides in rose petals. Analytes were extracted from the matrix using acetonitrile and a mixture of salts containing magnesium sulfate, sodium citrate, sodium chloride, and sodium sesquihydrate. Samples were cleaned up twice by d-SPE applying primary and secondary amines (PSAs), magnesium sulfate, C18, and graphitized carbon black (GCB). Two fortification levels of 0.05 and 0.5 mg kg(-1) were assessed for method validation purposes. The obtained pesticide recoveries were in the range of 70-120 % with a relative standard deviation (RSD) of less than 20 %. The newly developed method was allowed for the quantification of 57 pesticides residues. It was applied to pesticide residue detection in rose petals from an organic field, without treatment, compared to those from a field with classic phytosanitary treatment using fungicide and/or insecticide. We did not detect pesticide residues in rose petals from the organic field. The classically treated samples of roses contained pesticides such as chlorpyriphos and methidathion which are in accordance with the previous application of these pesticides on the roses. Insecticides were quantified at 0.05 mg kg(-1) rose petal maximum.