MilliporeSigma
  • Home
  • Search Results
  • Antibody-mediated immune suppression of erythrocyte alloimmunization can occur independently from red cell clearance or epitope masking in a murine model.

Antibody-mediated immune suppression of erythrocyte alloimmunization can occur independently from red cell clearance or epitope masking in a murine model.

Journal of immunology (Baltimore, Md. : 1950) (2014-08-15)
Honghui Yu, Sean R Stowell, Lidice Bernardo, Jeanne E Hendrickson, James C Zimring, Alaa Amash, Makoto Uchikawa, Alan H Lazarus
ABSTRACT

Anti-D can prevent immunization to the RhD Ag on RBCs, a phenomenon commonly termed Ab-mediated immune suppression (AMIS). The most accepted theory to explain this effect has been the rapid clearance of RBCs. In mouse models using SRBC, these xenogeneic cells are always rapidly cleared even without Ab, and involvement of epitope masking of the SRBC Ags by the AMIS-inducing Ab (anti-SRBC) has been suggested. To address these hypotheses, we immunized mice with murine transgenic RBCs expressing the HOD Ag (hen egg lysozyme [HEL], in sequence with ovalbumin, and the human Duffy transmembrane protein) in the presence of polyclonal Abs or mAbs to the HOD molecule. The isotype, specificity, and ability to induce AMIS of these Abs were compared with accelerated clearance as well as steric hindrance of the HOD Ag. Mice made IgM and IgG reactive with the HEL portion of the molecule only. All six of the mAbs could inhibit the response. The HEL-specific Abs (4B7, IgG1; GD7, IgG2b; 2F4, IgG1) did not accelerate clearance of the HOD-RBCs and displayed partial epitope masking. The Duffy-specific Abs (MIMA 29, IgG2a; CBC-512, IgG1; K6, IgG1) all caused rapid clearance of HOD RBCs without steric hindrance. To our knowledge, this is the first demonstration of AMIS to erythrocytes in an all-murine model and shows that AMIS can occur in the absence of RBC clearance or epitope masking. The AMIS effect was also independent of IgG isotype and epitope specificity of the AMIS-inducing Ab.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Diethanolamine, ACS reagent, ≥98.5%
Sigma-Aldrich
Anti-Mouse IgG (γ-chain specific)−Biotin antibody produced in goat, affinity isolated antibody, buffered aqueous solution
Sigma-Aldrich
Diethanolamine, reagent grade, ≥98.0%
Sigma-Aldrich
Diethanolamine, puriss. p.a., ACS reagent, ≥99.0% (GC)
Supelco
Diethanolamine, analytical standard
Sigma-Aldrich
Diethanolamine, BioUltra, ≥99.5% (GC)
Sigma-Aldrich
Anti-Mouse IgG (Fc specific), F(ab′)2 fragment, highly cross adsorbed-Biotin antibody produced in goat, affinity isolated antibody, lyophilized powder
Sigma-Aldrich
Anti-Mouse IgG F(ab′)2-Biotin antibody produced in goat, affinity isolated antibody, lyophilized powder
Trolamine impurity B, European Pharmacopoeia (EP) Reference Standard
Supelco
Diethanolamine, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Citrate-phosphate-dextrose solution with adenine
Sigma-Aldrich
Anti-Mouse IgG (whole molecule)−Biotin antibody produced in goat, affinity isolated antibody, buffered aqueous solution
Sigma-Aldrich
Anti-Mouse IgM (μ-chain specific)–Alkaline Phosphatase antibody produced in goat, affinity isolated antibody, buffered aqueous glycerol solution
Sigma-Aldrich
Anti-Mouse IgG (Fab specific)–Biotin antibody produced in goat, affinity isolated antibody, buffered aqueous solution
Sigma-Aldrich
Anti-Mouse IgG (Fc specific)–Biotin antibody produced in goat, affinity isolated antibody, buffered aqueous solution
Sigma-Aldrich
Anti-Mouse IgG (Fc specific)–Biotin antibody produced in goat, affinity isolated antibody, buffered aqueous solution
Sigma-Aldrich
Anti-Mouse IgG (whole molecule)−Biotin antibody produced in goat, affinity isolated antibody, buffered aqueous solution
Sigma-Aldrich
Anti-Mouse IgG (Fab specific)–Biotin antibody produced in goat, affinity isolated antibody, buffered aqueous solution
Sigma-Aldrich
Anti-Mouse IgG (H+L), Biotin antibody produced in goat, ~2 mg/mL, affinity isolated antibody
Sigma-Aldrich
Anti-Mouse IgG (H+L), highly cross-adsorbed, Biotin antibody produced in goat, ~2 mg/mL, affinity isolated antibody
Sigma-Aldrich
Anti-Mouse IgG F(ab′)2, F(ab′)2 fragment, highly cross absorbed-Biotin antibody produced in goat, affinity isolated antibody, lyophilized powder
Sigma-Aldrich
Anti-Mouse IgG (H+L), F(ab) fragment-Biotin antibody produced in goat, affinity isolated antibody, lyophilized powder
Sigma-Aldrich
Anti-Mouse IgG (H+L), F(ab′)2 fragment-Biotin antibody produced in goat, affinity isolated antibody, lyophilized powder