MilliporeSigma
  • Home
  • Search Results
  • Nestin expression and reactive phenomena in the mouse cochlea after kanamycin ototoxicity.

Nestin expression and reactive phenomena in the mouse cochlea after kanamycin ototoxicity.

The European journal of neuroscience (2014-04-03)
Tiziana Martone, Pamela Giordano, Federico Dagna, Daniela Carulli, Roberto Albera, Ferdinando Rossi
ABSTRACT

Following injury to the adult mammalian cochlea, hair cells cannot be spontaneously replaced. Nonetheless, the postnatal cochlea contains progenitor cells, distinguished by the expression of nestin, which are able to proliferate and form neurospheres in vitro. Such resident progenitors might be endowed with reparative potential. However, to date little is known about their behaviour in situ following hair cell injury. Using adult mice and ex vivo cochlear cultures, we sought to determine whether: (i) resident cochlear progenitors respond to kanamycin ototoxicity and compensate for it; and (ii) the reparative potential of cochlear progenitors can be stimulated by the addition of growth factors. Morphological changes of cochlear tissue, expression of nestin mRNA and protein and cell proliferation were investigated in these models. Our observations show that ototoxic injury has modest effects on nestin expression and cell proliferation. On the other hand, the addition of growth factors to the injured cochlear explants induced the appearance of nestin-positive cells in the supporting cell area of the organ of Corti. The vast majority of nestin-expressing cells, however, were not proliferating. Growth factors also had a robust stimulatory effect on axonal sprouting and the proliferative response, which was more pronounced in injured cochleae. On the whole, our findings indicate that nestin expression after kanamycin ototoxicity is related to tissue reactivity rather than activation of resident progenitors attempting to replace the lost receptors. In addition, administration of growth factors significantly enhances tissue remodelling, suggesting that cochlear repair may be promoted by the exogenous application of regeneration-promoting substances.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Monoclonal Anti-p27Kip1 antibody produced in mouse, clone DCS-72, ascites fluid
Sigma-Aldrich
Ampicillin, meets USP testing specifications
Sigma-Aldrich
Fluorescein isothiocyanate isomer I, ≥97.5% (HPLC)
Sigma-Aldrich
Fluorescein isothiocyanate isomer I, suitable for protein labeling, ≥90% (HPLC), powder
Sigma-Aldrich
Anti-CALB1 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Sigma-Aldrich
Monoclonal Anti-CDKN1B antibody produced in mouse, clone 4B4-E6, purified immunoglobulin, buffered aqueous solution
Sigma-Aldrich
Monoclonal Anti-MKI67 antibody produced in mouse, clone 7B8, purified immunoglobulin, buffered aqueous solution
Sigma-Aldrich
Anti-SOX2 antibody produced in rabbit, ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Sigma-Aldrich
Anti-NES antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Sigma-Aldrich
Fluorescein isothiocyanate isomer I, ≥97.5% (HPLC)
Sigma-Aldrich
Anti-NES antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Sigma-Aldrich
Anti-CDKN1B antibody produced in rabbit, affinity isolated antibody
Sigma-Aldrich
Anti-CALB1 antibody produced in rabbit, purified immunoglobulin, buffered aqueous solution
Sigma-Aldrich
Anti-SOX2 antibody produced in rabbit, affinity isolated antibody, buffered aqueous solution
Sigma-Aldrich
Xylazine, ≥99%
Sigma-Aldrich
Monoclonal Anti-MKI67 antibody produced in mouse, clone 8D5, ascites fluid
Sigma-Aldrich
Monoclonal Anti-MKI67 antibody produced in mouse, Prestige Antibodies® Powered by Atlas Antibodies, clone CL1234, purified immunoglobulin, buffered aqueous glycerol solution
Sigma-Aldrich
Monoclonal Anti-MKI67 antibody produced in mouse, clone 4A1, ascites fluid
Sigma-Aldrich
Monoclonal Anti-SOX2 antibody produced in mouse, clone 10F10, ascites fluid
Sigma-Aldrich
Monoclonal Anti-SOX2 antibody produced in mouse, clone 10F10C9, ascites fluid
Sigma-Aldrich
Anti-SOX2 antibody produced in rabbit, affinity isolated antibody
Sigma-Aldrich
Anti-Sox2 antibody, Mouse monoclonal, clone SOX2-6, purified from hybridoma cell culture
Sigma-Aldrich
Anti-MKI67 antibody produced in rabbit, Ab1, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Sigma-Aldrich
Anti-Nestin antibody produced in rabbit, ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Sigma-Aldrich
Anti-MKI67 antibody produced in rabbit, Ab2, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Sigma-Aldrich
Anti-NFASC antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody, buffered aqueous glycerol solution
Sigma-Aldrich
Fluorescein 5(6)-isothiocyanate, BioReagent, suitable for fluorescence, mixture of 2 components, ≥90% (HPLC)
Sigma-Aldrich
Ampicillin, anhydrous, 96.0-102.0% (anhydrous basis)
Sigma-Aldrich
β-D-Allose, rare aldohexose sugar
Sigma-Aldrich
DAPI, for nucleic acid staining