MilliporeSigma
  • Home
  • Search Results
  • Response to Nodal morphogen gradient is determined by the kinetics of target gene induction.

Response to Nodal morphogen gradient is determined by the kinetics of target gene induction.

eLife (2015-04-15)
Julien Dubrulle, Benjamin M Jordan, Laila Akhmetova, Jeffrey A Farrell, Seok-Hyung Kim, Lilianna Solnica-Krezel, Alexander F Schier
ABSTRACT

Morphogen gradients expose cells to different signal concentrations and induce target genes with different ranges of expression. To determine how the Nodal morphogen gradient induces distinct gene expression patterns during zebrafish embryogenesis, we measured the activation dynamics of the signal transducer Smad2 and the expression kinetics of long- and short-range target genes. We found that threshold models based on ligand concentration are insufficient to predict the response of target genes. Instead, morphogen interpretation is shaped by the kinetics of target gene induction: the higher the rate of transcription and the earlier the onset of induction, the greater the spatial range of expression. Thus, the timing and magnitude of target gene expression can be used to modulate the range of expression and diversify the response to morphogen gradients.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Monoclonal ANTI-FLAG® M2 antibody produced in mouse, clone M2, purified immunoglobulin (Purified IgG1 subclass), buffered aqueous solution (10 mM sodium phosphate, 150 mM NaCl, pH 7.4, containing 0.02% sodium azide)
Sigma-Aldrich
Cycloheximide, ≥95% (HPLC)
Supelco
Cycloheximide, PESTANAL®, analytical standard
Sigma-Aldrich
Cycloheximide, from microbial, ≥94% (TLC)
Sigma-Aldrich
Cycloheximide, Biotechnology Performance Certified
Sigma-Aldrich
Anti-SMAD2 (Ab-220) antibody produced in rabbit, affinity isolated antibody
Sigma-Aldrich
Anti-SMAD2 antibody produced in rabbit, affinity isolated antibody
Sigma-Aldrich
Anti-SMAD2 antibody produced in rabbit, affinity isolated antibody
Sigma-Aldrich
Anti-SMAD2 antibody produced in rabbit, affinity isolated antibody
Sigma-Aldrich
Anti-SMAD2 antibody produced in rabbit, affinity isolated antibody
Sigma-Aldrich
Anti-SMAD2 antibody produced in rabbit, affinity isolated antibody
Sigma-Aldrich
Anti-SMAD2, (N-terminal) antibody produced in rabbit, affinity isolated antibody
Sigma-Aldrich
Anti-SMAD2 antibody produced in rabbit, ~1.0 mg/mL, affinity isolated antibody
Sigma-Aldrich
Anti-SMAD2 (Ab-467) antibody produced in rabbit, affinity isolated antibody
Sigma-Aldrich
Cycloheximide solution, Ready-Made Solution, microbial, 100 mg/mL in DMSO, Suitable for cell culture
Sigma-Aldrich
SB-505124 hydrochloride hydrate, ≥98% (HPLC)
Millipore
Cycloheximide solution, 0.1%, suitable for microbiology