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  • The coronatine toxin of Pseudomonas syringae is a multifunctional suppressor of Arabidopsis defense.

The coronatine toxin of Pseudomonas syringae is a multifunctional suppressor of Arabidopsis defense.

The Plant cell (2012-12-04)
Xueqing Geng, Jiye Cheng, Anju Gangadharan, David Mackey
ABSTRACT

The phytotoxin coronatine (COR) promotes various aspects of Pseudomonas syringae virulence, including invasion through stomata, growth in the apoplast, and induction of disease symptoms. COR is a structural mimic of active jasmonic acid (JA) conjugates. Known activities of COR are mediated through its binding to the F-box-containing JA coreceptor CORONATINE INSENSITIVE1. By analyzing the interaction of P. syringae mutants with Arabidopsis thaliana mutants, we demonstrate that, in the apoplastic space of Arabidopsis, COR is a multifunctional defense suppressor. COR and the critical P. syringae type III effector HopM1 target distinct signaling steps to suppress callose deposition. In addition to its well-documented ability to suppress salicylic acid (SA) signaling, COR suppresses an SA-independent pathway contributing to callose deposition by reducing accumulation of an indole glucosinolate upstream of the activity of the PEN2 myrosinase. COR also suppresses callose deposition and promotes bacterial growth in coi1 mutant plants, indicating that COR may have multiple targets inside plant cells.

MATERIALS
Product Number
Brand
Product Description

Supelco
Indole, analytical standard
Supelco
Indole solution, 2000 μg/mL in methanol, certified reference material
Sigma-Aldrich
Indole, ≥99%
Sigma-Aldrich
Indole, ≥99%, FG