Ligand-directed tosyl chemistry for protein labeling in vivo.

Nature chemical biology (2009-03-31)
Shinya Tsukiji, Masayoshi Miyagawa, Yousuke Takaoka, Tomonori Tamura, Itaru Hamachi
ABSTRACT

Here we describe a method for the site-selective attachment of synthetic molecules into specific 'endogenous' proteins in vivo using ligand-directed tosyl (LDT) chemistry. This approach was applied not only for chemically labeling proteins in living cells, tissues and mice but also for constructing a biosensor directly inside cells without genetic engineering. These data establish LDT chemistry as a new tool for the study and manipulation of biological systems.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
L-Glutathione reduced, ≥98.0%
Sigma-Aldrich
L-Glutathione reduced, BioReagent, suitable for cell culture, ≥98.0%, powder
Sigma-Aldrich
L-Glutathione reduced, BioXtra, ≥98.0%
Sigma-Aldrich
Benzenesulfonamide, ≥98%
Sigma-Aldrich
4-Sulfamoylbenzoic acid, 97%