• Home
  • Search Results
  • Exercise-induced α-ketoglutaric acid stimulates muscle hypertrophy and fat loss through OXGR1-dependent adrenal activation.

Exercise-induced α-ketoglutaric acid stimulates muscle hypertrophy and fat loss through OXGR1-dependent adrenal activation.

The EMBO journal (2020-02-28)
Yexian Yuan, Pingwen Xu, Qingyan Jiang, Xingcai Cai, Tao Wang, Wentong Peng, Jiajie Sun, Canjun Zhu, Cha Zhang, Dong Yue, Zhihui He, Jinping Yang, Yuxian Zeng, Man Du, Fenglin Zhang, Lucas Ibrahimi, Sarah Schaul, Yuwei Jiang, Jiqiu Wang, Jia Sun, Qiaoping Wang, Liming Liu, Songbo Wang, Lina Wang, Xiaotong Zhu, Ping Gao, Qianyun Xi, Cong Yin, Fan Li, Guli Xu, Yongliang Zhang, Gang Shu

Beneficial effects of resistance exercise on metabolic health and particularly muscle hypertrophy and fat loss are well established, but the underlying chemical and physiological mechanisms are not fully understood. Here, we identified a myometabolite-mediated metabolic pathway that is essential for the beneficial metabolic effects of resistance exercise in mice. We showed that substantial accumulation of the tricarboxylic acid cycle intermediate α-ketoglutaric acid (AKG) is a metabolic signature of resistance exercise performance. Interestingly, human plasma AKG level is also negatively correlated with BMI. Pharmacological elevation of circulating AKG induces muscle hypertrophy, brown adipose tissue (BAT) thermogenesis, and white adipose tissue (WAT) lipolysis in vivo. We further found that AKG stimulates the adrenal release of adrenaline through 2-oxoglutarate receptor 1 (OXGR1) expressed in adrenal glands. Finally, by using both loss-of-function and gain-of-function mouse models, we showed that OXGR1 is essential for AKG-mediated exercise-induced beneficial metabolic effects. These findings reveal an unappreciated mechanism for the salutary effects of resistance exercise, using AKG as a systemically derived molecule for adrenal stimulation of muscle hypertrophy and fat loss.

Product Number
Product Description

MISSION® esiRNA, targeting human OXGR1
Sodium pyruvate solution, 100 mM, sterile-filtered, BioReagent, suitable for cell culture
Triton X-100, BioXtra
D-(+)-Glucose solution, 45% in H2O, sterile-filtered, BioXtra, suitable for cell culture
Triton X-100, laboratory grade
SR 59230A, ≥98% (HPLC), powder
3,3′-Diaminobenzidine, 98%
L-Glutamine, meets USP testing specifications, suitable for cell culture, 99.0-101.0%, from non-animal source
Docosanoic acid tryptamide, analytical standard
Anti-HSPG2 antibody produced in rabbit, affinity isolated antibody
Anti-OXGR1 antibody produced in rabbit, Prestige Antibodies® Powered by Atlas Antibodies, affinity isolated antibody