MilliporeSigma

Duolink® Proximity Ligation Assay

Duolink® Proximity Ligation Assay (PLA)

Inside every cell, there is a breakthrough waiting to be uncovered. The chance to change the origin of a human disease or discover the tell-tale signal of an irregular protein-protein interaction requires powerful investigative tools and a streamlined approach. Rely on Duolink® PLA to amplify protein signals by 1000x, allowing you to visually detect proteins and their interactions, all with a straightforward protocol.

Uncover the opportunities in your research with amplified performance and the benefits of Duolink® PLA technology:

  • Visualize protein interactions – both stable and transient
  • Detect endogenous proteins – no overexpression or genetic manipulation
  • High specificity – use of two antibodies/probes eliminates false positives
  • Single molecule sensitivity – rolling circle amplification makes proteins visible
  • No special equipment needed – standard immunofluorescence methods
  • Publication-ready results – compare image results from indirect methods (Co-IP/WB)



Protein-Protein Interaction Detection and Quantification

Proteins influence cellular function by interacting with other proteins, DNA, membrane components, structural components, etc. Duolink® PLA allows you to see these interactions within the cell, with clear, visual signals. The signal is generated only if the proteins of interest are within 40nm, therefore detecting the interaction.

Post-Translational Modification (PTM) Detection and Quantification

Reliably detecting protein modifications, such as methylation or phosphorylation, can be challenging. Duolink® PLA allows you to detect PTMs with specificity and sensitivity. Use two antibodies, one for the protein, and one for its modification of interest. If the modification is present on the protein, then both antibodies should bind and generate a signal.

Low Expression Protein Detection and Quantification

Low abundance proteins and biomarkers can be missed due to lack of detection sensitivity with traditional techniques. Duolink® PLA allows you to detect as few as a single event. As long as you have the target specific antibodies, you will detect the protein. No overexpression or genetic manipulation is needed.

Duolink® Probemaker Kits

For experiments where the Duolink® PLA Probes are not an option because the host species of a primary antibody is different than mouse, rabbit or goat, Duolink® Probemaker kits enable the conjugation of a PLA oligo (PLUS or MINUS) to any antibody. Antibody conjugation with Duolink® Probemaker is quick, easy and requires no clean-up. Discover Duolink® Probemaker and create your own PLA® probes.

Duolink® PLA Multicolor Kits

For use in a multiplex application, allowing for endogenous, simultaneous detection of up to 4 protein interactions, post-translational modifications, or protein expression levels (including low abundant protein) in fixed cells and tissue samples, the Duolink® PLA Multicolor Probemaker Kits are available in four different colors: red, green, orange and far red. Each Multicolor Probemaker kit contains all the necessary reagents to perform the conjugations of unique pairs of oligos directly to a pair of primary antibodies. The resulting PLA probes must be used together and cannot be paired with any other Duolink® PLA probe.