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G006128

HPLC Analysis of Proteins by Size Exclusion on Zenix® SEC-300 Columns, Effect of Flow Rate (30 cm column)

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Zenix SEC-300, 30 cm x 4.6 mm I.D., 3 μm, 300 Å particles (Z777028)
mobile phase: 150 mM potassium phosphate, pH 6.8
flow rate: as indicated on figure
column temp.: ambient
detector: UV, 280 nm
injection: 10 μL
sample: each 1 mg/mL except uracil, 2.5 mg/mL

suitability

application for HPLC

application(s)

life science and biopharma
pharmaceutical (small molecule)

Legal Information

Zenix is a registered trademark of Sepax Technologies

Analyte

Description
    Thyroglobulin from bovine thyroid, powder, ≥90% (agarose gel electrophoresis)
    Glucose-6-phosphate Dehydrogenase from baker’s yeast (S. cerevisiae), Type XV, lyophilized powder, 200-400 units/mg protein (modified Warburg-Christian)
    Bovine Serum Albumin, lyophilized powder, ≥96% (agarose gel electrophoresis)
    Ribonuclease A from bovine pancreas, for molecular biology, ≥70 Kunitz units/mg protein, lyophilized
    Uracil, ≥99.0% (T)

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