G005922

HPLC Analysis of Aflatoxins B1, B2, G1 and G2 in Corn on Ascentis^® Express C18 after SPE using Supel^™ Tox AflaZea and Fluorescence Detection

application for HPLC

Properties

• technique(s): HPLC: suitable
• test parameters: sample preparation: SPE (Solid Phase Extraction); sample/matrix: 25 g corn meal in 100 mL acetonitrile:water (84:16); SPE tube/cartridge: Supel^™ Tox AflaZea, 6 mL (55314-U); sample addition: 2 mL; elution: collect sample in 5 mL silanized glass vial; eluate post-treatment: transfer 200 μL sample, add 300 μL TFA derivatization solution (70:20:10 water:TFA:acetic acid), heat in microreactor 20 min at 65 °C, cool, add 580 μL deionized water, vortex; column: Ascentis Express C18, 15 cm x 2.1 mm I.D., 2.7 μm particles (53825-U); mobile phase: [A] water; [B] acetonitrile; (80:20, A:B); flow rate: 0.350 mL/min; pressure: 325 psi (22.4 bar); column temp.: 35 °C; detector: fluorescence, ex 360 nm, em 440 nm; injection: 20 μL
• suitability: application for HPLC
• application(s): food and beverages

Description

Analysis Note

Aflatoxins are closely related compounds produced as secondary metablolites of fungi. They are found in grains and nuts like corn, wheat or peanuts. Shown here is a rapid, sensitive method to extract these compounds from corn using SPE cartridges specifically designed for aflatoxins, Supel Tox AflaZea (aflatoxin zearalenone), which removes interferences from grains, feeds, TMR samples, peanuts, peanut products and aqueous solutions. Analysis was via HPLC on Ascentis Express Fused-Core C18 colums.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Supel is a trademark of Sigma-Aldrich Co. LLC

Analyte

Aflatoxin G₁ solution, 3 μg/mL in benzene:acetonitrile (98:2), analytical standard
Aflatoxin B₁ solution, 20 μg/mL in methanol, analytical standard
Aflatoxin G₂ solution, 3 μg/mL in benzene:acetonitrile (98:2), analytical standard
Aflatoxin B₂ solution, 3 μg/mL in benzene:acetonitrile (98:2), analytical standard