G005748
HPLC Analysis of Tamoxifen E/Z isomers and Related Compounds on Ascentis® Express RP-Amide
application for HPLC
About This Item
technique(s)
HPLC: suitable
test parameters
column: Ascentis Express RP-Amide, 10 cm x 3 mm I.D., 2.7 μm particles (53918-U)
mobile phase: [A] 0.1% formic acid in water; [B] 0.1% formic acid in acetonitrile
gradient: held at 25% B for 2 min; 25 to 40% B in 15 min; 40 to 25% B in 0.1 min; held at 25% B for 2.9 min
flow rate: 0.6 mL/min
pressure: 2292 psi (158 bar)
column temp.: 35 °C
detector: UV, 260 nm
injection: 2 μL
sample: 10 mg/L in 10:90, water: methanol
suitability
application for HPLC
application(s)
pharmaceutical (small molecule)
Legal Information
Analyte
analytical column
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