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G005680

HPLC Analysis of Biogenic Amines on Ascentis® RP-Amide

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Ascentis RP-Amide, 15 cm x 2.1 mm I.D., 5 μm particles (565305-U)
mobile phase: [A] 0.1% ammonium acetate in water (pH unadjusted); [B] acetonitrile
gradient: 25 to 75% B in 10 min; held at 75% B for 5 min
flow rate: 0.5 mL/min
column temp.: 35 °C
detector: UV, 340 nm
injection: 10 μL

suitability

application for HPLC

application(s)

clinical

Analysis Note

Following pre-column derivatization of primary amino groups using o-phthaldialdehyde/thiol reagent, the Ascentis RP-Amide was used for the analysis of biogenic amines commonly found in wines. These include histamine, spermine, spermidine, tyramine, putrescine and cadaverine. Structures along with an optimized chromatogram are presented.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Histamine
    Spermine
    Spermidine
    Tyramine
    1,4-Diaminobutane
    Cadaverine

Standard

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Description
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analytical column

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