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G005650

HPLC Analysis of Synthetic Food Dyes on Ascentis® Express C18

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.7 μm particles (53823-U)
mobile phase: [A] 100 mM ammonium phosphate monobasic, pH 7.0 with ammonium hydroxide; [B] water; [C] acetonitrile
gradient: 10% A constant; 85 to 25% B, 5 to 65% C in 6 min; 25% B, 65% C held for 5 min
flow rate: 0.3 mL/min
column temp.: 40 °C
detector: UV, 254 nm
injection: 5 μL
sample: 20 μg/mL each in water

suitability

application for HPLC

application(s)

food and beverages

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

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analytical column

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