Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

G005634

HPLC Analysis of Drugs of Abuse on Ascentis® Express F5

application for HPLC

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53569-U)
mobile phase: [A] 5 mM ammonium formate; [B] 5 mM ammonium formate in 95:5 (v/v) acetonitrile:water
gradient: held at 20% B for 0.5 min; 20 to 100% B in 5.5 min; held at 100% B for 4 min
flow rate: 0.3 mL/min
pressure: 175 bar
column temp.: 35 °C
detector: ESI(+), m/z 100-1000
injection: 1 μL
sample: 1 μg/mL in 50:50, water: methanol

suitability

application for HPLC

application(s)

forensics and toxicology

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Benzoylecgonine
    Morphine
    Codeine
    Oxycodone
    Hydrocodone
    (±)-Deoxyephedrine hydrochloride
    Fentanyl

Analytical column

Product No.
Description
Pricing

Standard

Product No.
Description
Pricing

C5901

Codeine monohydrate, analytical standard

44179

Morphine sulfate salt pentahydrate, analytical standard

B-007

Benzoylecgonine solution, 100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

F-002

Fentanyl solution, 100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

H-003

Hydrocodone solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

M-013

(±)-MDMA solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

M-022

(±)-Methamphetamine solution, 100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

M-035

Meperidine solution, 1.0 mg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

N-017

Normeperidine solution, 100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant®

P-011

(+)-Propoxyphene solution, 1.0 mg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant®

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Sorry, we don't have COAs for this product available online at this time.

If you need assistance, please contact Customer Support.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service