G005593

HPLC Analysis of Flavonoids in Lime Juice on Ascentis^® Express C18

application for HPLC, application for SPE

Properties

• technique(s): HPLC: suitable; solid phase extraction (SPE): suitable
• test parameters: sample preparation: SPE (Solid Phase Extraction); sample/matrix: lime juice hand-squeezed in laboratory, centrifuged and filtered through a 0.45 μm nylon filter disc (Acrodisc); column: Ascentis Express C18, 5 cm x 4.6 mm I.D., 2.7 μm particles (53826-U); mobile phase: [A] water/formic acid (99.9:0.1); [B] water/acetonitrile/isopropanol/formic acid (39.9:20:40:0.1); gradient: 10% B for 3 min; 10 to 34% B in 30 min; 34 to 100% B in 1 min; held at 100% B for 6 min; 100 to10% B in 1 min; flow rate: 0.8 mL/min; column temp.: ambient; detector: PDA: 190–370 nm; chromatogram extracted at 283 nm; detector: ESI-MS: mass spectral range, 250-700 m/z; interval, 0.5 s; scan speed, 938 amu/s; nebulizing gas (N2) flow, 1.5 L/min; (interface temperature, 350 °C; heat block, 300 °C; desolvation line (DL) temperature, 300 °C; DL voltage, 34 V; probe voltage, +4.5 kV; Qarray voltage, 1.0 V; RF voltage, 90 V; detection gain, 1.05 kV); injection: 2 μL
• suitability: application for HPLC; application for SPE
• application(s): food and beverages

Description

Analysis Note

Flavonoids are polyphenolic compounds ubiquitous in nature and classified, according to their chemical structure, into flavonols, flavones, flavanones, isoflavones, catechins, anthocyanidins and chalcones. They are widely present in fruits, vegetables, leaves, nuts, seeds, bark and beverages (tea, coffee, beer, wine and fruit drinks).

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

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