G005446

HPLC Analysis of Spice Cannabinoids on Ascentis^® Express F5

application for HPLC

Properties

• technique(s): HPLC: suitable
• test parameters: column: Ascentis Express F5, 10 cm x 2.1 mm I.D., 2.7 μm particles (53569-U); mobile phase: [A] 50 mM ammonium formate; [B] water; [C] acetonitrile; (10:35:55, A:B:C); flow rate: 0.6 mL/min; pressure: 4075 psi (281 bar); column temp.: 30 °C; detector: UV, 200 nm; injection: 3 μL; sample: 100 μg/mL in 45:55 water:acetonitrile
• suitability: application for HPLC
• application(s): forensics and toxicology

Description

Analysis Note

Synthetic cannabinoids (e.g. "Spice") are a type of designer drug that provide a cannabis-type high. New synthetic cannabinoids are continually being introduced as suppliers tweak the molecular structures. The ability to rapidly and reliably identify the continually changing population of these compounds is a significant analytical challenge facing forensic chemists. A rapid separation of eight of these compounds on Ascentis Express F5 column is shown here. Cerilliant CRMs provided reliable identification and quantification.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

JWH-073 N-(3-hydroxybutyl) metabolite solution, 100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant^®
HU-210 solution, 100 μg/mL in methanol, ampule of 1 mL, (Spice Cannabinoid), certified reference material, Cerilliant^®
JWH-018 solution, 100 μg/mL in methanol, ampule of 1 mL, certified reference material, Cerilliant^®
JWH-073 solution, 100 μg/mL in acetonitrile, ampule of 1 mL, certified reference material, Cerilliant^®
Spice Cannabinoid Mix solution, 100 μg/mL each component in acetonitrile, ampule of 1.0 mL, certified reference material, Cerilliant^®