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G004647

HPLC Analysis of Pyroglutethimide Enantiomers on Astec® CHIROBIOTIC® V

application for HPLC

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technique(s)

HPLC: suitable

test parameters

column: CHIROBIOTIC V, 25 cm x 4.6 mm I.D., 5 μm particles (11024AST)
mobile phase: [A] 0.1% triethylamine acetate, pH 7.0; [B] acetonitrile; (90:10, A:B)
flow rate: 1 mL/min
column temp.: 25 °C
detector: UV, 254 nm
injection: 3 μL
sample: 2 mg/mL in methanol

suitability

application for HPLC

application(s)

pharmaceutical (small molecule)

Legal Information

Astec is a registered trademark of Merck KGaA, Darmstadt, Germany
CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC

Analytical column

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