85-438

HPLC Analysis of Nonvolatile Pesticides in Water on SUPELCOSIL^™ LC-18-DB after SPE using Supelclean^™ ENVI-Carb^™

application for SPE, application for HPLC

Properties

• technique(s): HPLC: suitable; solid phase extraction (SPE): suitable
• test parameters: sample preparation: SPE (Solid Phase Extraction); sample/matrix: water spiked with pesticides at 10-50 μg/L; SPE tube/cartridge: Supelclean ENVI-Carb, 250 mg/3 mL (57088); condition: 5 mL methylene chloride:methanol (80:20); 1 mL methanol; 10 mL 2% acetic acid in water (keep bed moist until sample addition); sample addition: 100 mL sample at 5 mL/min; drying: 1 minute with vacuum; elution: 0.8 mL methanol; 2 x 35 mL methylene chloride:methanol (80:20); eluate post-treatment: dry eluate to 500 μL under gentle nitrogen stream, reconstitute to 1 mL with methanol; column: SUPELCOSIL LC-18-DB, 25 cm × 4.6 mm I.D., 5 μm particles (58355-U); mobile phase: [A] water:acetonitrile (90:10), [B] acetonitrile; gradient 80% A for 5 min then to 30% A over 30 min; flow rate: 1.5 mL/min; detector: UV, 220 nm; injection: 20 μL
• suitability: application for HPLC; application for SPE
• application(s): environmental

Description

Legal Information

ENVI-Carb is a trademark of Sigma-Aldrich Co. LLC

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC

Supelclean is a trademark of Sigma-Aldrich Co. LLC

Analyte

Monuron
Propachlor
Linuron
Aldicarb
N-(1-Naphthyl)thiourea
Carbofuran
Cyanazine
Metribuzin
Atrazine
Propham
Methomyl
Diuron
1-Naphthyl-N-methylcarbamate
Simazine
See All (14)