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TOYOPEARL® Phenyl-650M Bulk Media

matrix polymer (65 μm), bottle of 100 mL, 65 μm

Synonym(s):

TOYOPEARL® Phenyl-650 Bulk Media

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About This Item

UNSPSC Code:
41115700

description

Phenyl-650M

product line

TOYOPEARL®

form

slurry

packaging

bottle of 100 mL

parameter

≤50% organic solvent
3 bar max. pressure

technique(s)

HPLC: suitable

matrix

polymer (65 μm)

matrix active group

phenyl phase

particle size

65 μm

operating pH

1-13

separation technique

hydrophobic interaction (HIC)

General description

Toyopearl Phenyl-650 resin is the middle hydrophobicity of the five HIC ligands offered by Tosoh Bioscience. Primary applications are for the separation of proteins, their isoforms, and aggregate removal. It is typically used in intermediate purification and polishing steps. For high performance separations, the use of TSKgel Phenyl-5PW resin is suggested. Both products have the same selectivity.

Application

Toyopearl media are used in separation media and resins. Toyopearl media offer high yield recovery of proteins, using various aqueous eluants.

Specifications

Clean in place with 0.5 M NaOH or 0.1 M HCl.

Physical form

Shipped in 20% (v/v) ethanol.

Recommended products

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

Toyopearl is a registered trademark of Tosoh Corporation

pictograms

Flame
GHS02

signalword

Warning

hcodes

H226

Hazard Classifications

Flam. Liq. 3

wgk_germany

WGK 1

ppe

Eyeshields, Gloves, type N95 (US)

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Tara Tibbs Jones et al.
Biotechnology and bioengineering, 87(3), 388-399 (2004-07-29)
Interesting retention and selectivity changes have been noted for a number of proteins in hydrophobic interaction chromatography (HIC). In this study, we investigated the degree to which conformational changes may be responsible for selectivity changes of stable proteins. Hydrogen-deuterium isotope
V Stocchi et al.
Journal of chromatography. A, 676(1), 51-63 (1994-07-29)
Hydrophobic interaction chromatography (HIC) has been employed extensively in the separation of proteins by elution using a descending salt gradient, with and without the use of detergents or denaturing agents. In this study, a new hydrophobic interaction chromatographic support, Toyopearl
P Ceccaroli et al.
Journal of chromatography. B, Biomedical sciences and applications, 702(1-2), 41-48 (1998-02-04)
Hydrophobic interaction chromatography (HIC) has been used extensively for the separation of proteins and peptides by elution using a descending salt gradient, with and without the use of detergents or denaturing agents. In this paper we compare different hydrophobic interaction

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