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814471

TOYOPEARL® AF-Tresyl-650M Bulk Media

phase tresyl-activated, bottle of 5 g, 65 μm particle size

Synonym(s):

TOYOPEARL® AF-Tresyl-650M Bulk Media

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About This Item

UNSPSC Code:
41115700

description

AF-Tresyl-650M

product line

TOYOPEARL®

form

powder

packaging

bottle of 5 g

manufacturer/tradename

Tosoh TOYOPEARL® AF, 14471

extent of labeling

80 μmol/g dry resin ligand density

parameter

3 bar max. pressure

technique(s)

HPLC: suitable

matrix

hydroxylated methacrylic polymer particle platform

matrix active group

tresyl-activated phase

particle size

65 μm

pore size

1000 Å

separation technique

affinity

storage temp.

2-8°C

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General description

Toyopearl AF-Tresyl-650 resin is an activated resin which readily binds to amine and thiol groups. The resin is provided dry for introduction into a buffered solution containing protein or other ligand in neutral to slightly alkaline (pH 7-8) conditions.

Application

TOYOPEARL® Affinity Media are used in affinity chromatography, protein chromatography and activated/functionalized matrices.

Physical form

Shipped in dry, free flowing powder.

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Legal Information

Toyopearl is a registered trademark of Tosoh Corporation

Related product

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Description
Pricing

pictograms

Exclamation mark
GHS07

signalword

Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
026H0208
026H0207

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S Knör et al.
Journal of thrombosis and haemostasis : JTH, 6(3), 470-477 (2008-01-16)
Hemophilia A is currently treated by infusions of the coagulation factor (F) VIII, of which production and purification remain a challenging task. Current purification procedures using immunoaffinity chromatography are cumbersome, expensive, and suffer from the instability of the applied antibody
S Yamauchi et al.
The Journal of biological chemistry, 274(4), 2456-2463 (1999-01-16)
Chondroitin 4-sulfotransferase, which transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate to position 4 of N-acetylgalactosamine in chondroitin, was purified 1900-fold to apparent homogeneity with 6.1% yield from the serum-free culture medium of rat chondrosarcoma cells by affinity chromatography on heparin-Sepharose CL-6B, Matrex
Zhenzhen Wen et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 879(20), 1732-1740 (2011-05-17)
In order to improve its stability, immobilized Concanavalin A (Con A) on Toyopearl adsorbents was conjugated with monomethoxy poly(ethylene glycol) succinimidyl propionate (mPEG-SPA) with different molecular weight. A colorimetric method using ninhydrin is proposed to determine the degree of PEGylation;

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