796-0128

GC Analysis of Pesticides in Fruits and Vegetables on a 14% Cyanopropylphenyl Column after SPE using Supelclean ENVI-Carb^™

suitable for GC, application for SPE

Properties

• technique(s): gas chromatography (GC): suitable; solid phase extraction (SPE): suitable
• test parameters: sample preparation: SPE (Solid Phase Extraction); sample/matrix: extract from 50 g homogenized fruit or vegetable sample; SPE tube/cartridge: ENVI-Carb, 500 mg/6 mL (57094); sample addition: 0.5 mL concentrated sample extract; elution: 20 mL acetonitrile:toluene (3:1); eluate post-treatment: Concentrate to 2 mL by rotary evaporation. Add 2 x 10 mL acetone concentrating to 2 mL after each acetone addition. (Add 50 μL I.S. (500 ng/μL cis-chlordane in acetone) then dilute to 2.5 mL with acetone.); column: 14% cyanopropylphenyl polysiloxane, 30m × 0.25 mm I.D., 0.15 μm (Supelco equivalent, Equity-1701, 28372-U); oven: 70 °C (hold 2 min) to 130 °C at 25 °C; 130 °C to 220 °C at 2 °C/min; 220 °C to 280 °C at 10 °C/min and hold 4.6 min; detector: MSD, 285 °C; carrier gas: helium; injection: 2 μL, splitless
• suitability: application for SPE
• application(s): agriculture; food and beverages

Description

Application

Sample Pre-treatment:
Homogenize 50 g chopped fruit/vegetable sample in 100 mL acetonitrile. Add 10 g sodium chloride and homogenize 5 min. Transfer 13 mL of acetonitrile layer to clean centrifuge tube. Centrifuge at high speed for 5 min. Evaporate 10 mL aliquot of supernatant to 0.5 mL under Nitrogen at 35°C.

Legal Information

ENVI-Carb is a trademark of Sigma-Aldrich Co. LLC