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Ascentis® ES-Cyano (5 µm) HPLC Columns

L × I.D. 25 cm × 4.6 mm, HPLC Column

Synonym(s):

Cyano Bonded Phase Column

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About This Item

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52

product name

Ascentis® ES-Cyano HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm

material

stainless steel column

Quality Level

100

agency

suitable for USP L10

product line

Ascentis®

feature

endcapped

manufacturer/tradename

Ascentis®

packaging

1 ea of

extent of labeling

10% Carbon loading

parameter

≤70 °C temp. range
400 bar pressure (5801 psi)

technique(s)

HPLC: suitable
LC/MS: suitable

L × I.D.

25 cm × 4.6 mm

surface area

450 m2/g

surface coverage

2.5 μmol/m2

impurities

<5 ppm metals

matrix

fully porous particle
silica gel high purity, spherical

matrix active group

cyano phase

particle size

5 μm

pore size

100 Å

operating pH

1-8

application(s)

food and beverages

separation technique

hydrophilic interaction (HILIC)
normal phase
reversed phase

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General description

Extra stable for low pH mobile phases due to sterically protected phase.

Useful for selectivity in the reversed-phase mode, including π π and dipole-dipole interacting compounds. Can also be used HILIC mode and normal phase chromatography.

Features and Benefits

  • Enhanced stability at low pH
  • Operates in reversed-phase, HILIC, and normal phase modes of chromatography
  • Low MS bleed
  • 100% aqueous compatible
  • Available as 3 μm and 5 μm particles
  • Particle composition: Type B silica gel
  • Particle shape: Spherical

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Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

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Certificates of Analysis (COA)

Lot/Batch Number
282925-02
282104-02
282104-01
280884-02
280884-01

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Meiyun Shi et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 951-952, 129-134 (2014-02-22)
Aralia mandshrica is a well-known traditional Chinese medicine from Northeast China commonly used to treat digestive, circulatory and immune system disorders. Calenduloside E is one of its bioactive components currently under evaluation as a pure drug. In this study, a
Alex D Batista et al.
Talanta, 133, 142-149 (2014-12-02)
On-line sample pretreatment (clean-up and analyte preconcentration) is for the first time coupled to sequential injection chromatography. The approach combines anion-exchange solid-phase extraction and the highly effective pentafluorophenylpropyl (F5) fused-core particle column for separation of eight sulfonamide antibiotics with similar
Jakub Fibigr et al.
Journal of pharmaceutical and biomedical analysis, 120, 112-119 (2016-01-01)
A new high-performance liquid chromatography method using fused-core column for fast separation of resveratrol and polydatin has been developed and used for quality control of nutraceuticals with resveratrol and polydatin content. Retention characteristics (log k) were studied under different conditions
Jan Soukup et al.
Journal of chromatography. A, 1374, 102-111 (2014-12-30)
Excess adsorption of water from aqueous acetonitrile mobile phases was investigated on 16 stationary phases using the frontal analysis method and coulometric Karl-Fischer titration. The stationary phases include silica gel and silica-bonded phases with different polarities, octadecyl and cholesterol, phenyl

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