ZRB3127
Anti-CHD4 Antibody, clone 1C17 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
ATP-dependent helicase CHD4, CHD-4, Chromodomain-helicase-DNA-binding protein 4, Mi-2 autoantigen 218 kDa protein
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About This Item
UNSPSC Code:
12352203
biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
1C17, recombinant monoclonal
description
recombinant, expressed in HEK 293 cells
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 218 kDa
observed mol wt ~240 kDa
purified by
using Protein A
species reactivity
mouse, human, rat
species reactivity (predicted by homology)
bovine, sheep, monkey, feline, horse
packaging
antibody small pack of 25
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)
affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG
epitope sequence
N-terminal half
Protein ID accession no.
UniProt accession no.
shipped in
ambient
storage temp.
2-8°C
Gene Information
human ... CHD4(1108)
General description
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 1C17 is a ZooMAb® rabbit recombinant monoclonal antibody that detects CHD4. It targets an epitope within 21 amino acids from the N-terminal half.
Immunogen
KLH-conjugated linear peptide corresponding to 21 amino acids from the N-terminal half of human CHD4.
Application
Quality Control Testing
Evaluated by Western Blotting in Raji cell nuclear extract.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected CHD4 in Raji cell nuclear extract.
Tested Applications
Western Blotting Analysis: A 1:1,000-10,000 dilution from a representative lot detected CHD4 in lysates from C6 and NIH/3T3 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CHD4 in human stomach cancer tissue sections and human small intestine tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CHD4 in NIH/3T3 cells.
Affinity Binding Assay: A representative lot of this antibody bound with CHD4 peptide at least ten thousand-fold (10,000X) higher than with the non-specific control peptide.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in Raji cell nuclear extract.
Western Blotting Analysis: A 1:10,000 dilution of this antibody detected CHD4 in Raji cell nuclear extract.
Tested Applications
Western Blotting Analysis: A 1:1,000-10,000 dilution from a representative lot detected CHD4 in lysates from C6 and NIH/3T3 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected CHD4 in human stomach cancer tissue sections and human small intestine tissue sections.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CHD4 in NIH/3T3 cells.
Affinity Binding Assay: A representative lot of this antibody bound with CHD4 peptide at least ten thousand-fold (10,000X) higher than with the non-specific control peptide.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Target description
Chromodomain-helicase-DNA-binding protein 4 (UniProt: Q14839; EC:3.6.4.12; also known as CHD-4, ATP-dependent helicase CHD4, Mi-2 autoantigen 218 kDa protein, Mi2-beta) is encoded by the CHD4 gene (Gene ID: 1108) in human. CHD4 is a member of the CHD family of proteins, which are characterized by the presence of chromo domains, helicase domains, and DNA-binding domains. CHD4 is a central component of the nucleosome remodeling and deacetylase (NuRD) complex, regulating gene expression, involved in early B cell development, in neuronal differentiation and the repair of DNA double-strand breaks by facilitating chromatin relaxation at damage sites. CHD4 is expressed in various tissues, with particularly high during embryonic development. CHD4 has been implicated in several diseases. Mutations and dysregulation of CHD4 are associated with various cancers, including breast, ovarian, and colorectal cancers. It is linked to intellectual disabilities and developmental delay, highlighting its importance in brain development. Aberrant CHD4 activity can lead to immune dysregulation and contribute to autoimmune diseases. CHD4 dysfunction has been associated with congenital heart defects and other cardiovascular anomalies. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Hagman, J. R., (2022) Immunological Reviews, 305. 29 42)
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Reconstitution
300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
11 - Combustible Solids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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