ZRB2594

Anti-Lactotransferrin Antibody, clone 1D15 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Growth-inhibiting protein 12, Talalactoferrin
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: recombinant monoclonal
• description: clone 1D15 ; recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 78.18 kDa; observed mol wt ~80 kDa
• purified by: using Protein A
• species reactivity: human
• packaging: antibody small pack of 25
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: Internal
• Protein ID accession no.: NP_002334
• UniProt accession no.: P02788
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... LTF(4057)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1D15 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Lactotransferrin. It targets an epitope within 16 amino acids from the internal region.

Immunogen

KLH-conjugated linear peptide corresponding to 16 amino acids from the internal region of human Lactotransferrin.

Application

Quality Control Testing

Evaluated by Western Blotting in Human prostate tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Lactotransferrin in Human prostate tissue lysate.

Tested Applications

Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Lactotransferrin in Human salivary gland tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Lactotransferrin in HepG2 cells.

Affinity Binding Assay: A representative lot of this antibody bound Lactotransferrin peptide with a KD of 9.6 x 10-8 in an affinity binding assay.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Lactotransferrin in Human salivary gland tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Lactotransferrin (UniProt: P02788; also known as Growth-inhibiting protein 12, Talalactoferrin, LF) is encoded by the LTF (also known as GIG12, LF) gene (Gene ID: 4057) in human. Lactotransferrin is an iron-binding, monomeric, glycoprotein found in exocrine fluids, including in breast milk and mucosal secretions. It plays an important role in the human innate immune system. Three different isoforms of lactotransferrin (LF) have been described: LF- is the iron-binding form and lacks ribonuclease activity; LF-β and LF- show ribonuclease activity but do not display iron-binding capacity. It inhibits bacterial growth by sequestering essential iron. It also exhibits non-iron-dependent antibacterial, antifungal, antiviral, antitumor, anti-inflammatory, and immunoregulatory activities. It also exhibits direct bactericidal properties leading to the release of lipopolysaccharides from the bacterial outer membrane. It is reported to stimulate the TLR4 signaling pathway leading to NF-kB activation and subsequent pro-inflammatory cytokine production while also interfering with the lipopolysaccharide (LPS)-stimulated TLR4 signaling. LF also has anabolic, differentiating, and anti-apoptotic effects on osteoblasts and can inhibit osteoclastogenesis, possibly playing a role in the regulation of bone growth. It is reported to have anti-tumor and anti-metastatic activity in different cancers. LTF gene is significantly down-regulated in nasopharyngeal carcinoma (NPC) and is negatively associated with the progression and metastasis of NPC. LF can suppress PDK1 expression via the MAP kinase/c-Jun pathway and repress AKT signaling. It can also interact with keratin 18 and block the formation of the K18 14-3-3 complex, leading to downregulation of K18-mediated AKT activation. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonal. (Ref.: Deng, M., et al. (2013). Oncogene. 32(36); 4273-4283; Ando, K., et al. (2010). FEBS J. 277(9); 2051-2066; Cornish, J., et al. (2004). Endocrinology. 145(9); 4366-4374; Arnold, RR., et al. (1982). Infect. Immun. 35(3); 792-799).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable