ZRB2538
Anti-TRIM14 Antibody, clone 1J17 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
Tripartite motif-containing protein 14
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About This Item
UNSPSC Code:
12352203
biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
1J17, recombinant monoclonal
description
recombinant, expressed in HEK 293 cells
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 49.77 kDa
observed mol wt ~50 kDa
purified by
using Protein A
species reactivity
human
packaging
antibody small pack of 25
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)
affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG
epitope sequence
N-terminal half
Protein ID accession no.
UniProt accession no.
shipped in
ambient
storage temp.
2-8°C
Gene Information
human ... TRIM14(9830)
General description
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 1J17 is a ZooMAb® recombinant rabbit monoclonal antibody that specifically detects TRIM14. It targets an epitope within 21 amino acids from the N-terminal half.
Immunogen
KLH-conjugated linear peptide corresponding to 21 amino acids from the N-terminal half of human TRIM14.
Application
Quality Control Testing
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected TRIM14 in HeLa cell lysate.
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected TRIM14 in human liver tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound TRIM14 peptide with at least one thousand-fold (1,000X) higher than with non-specific control peptide.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected TRIM14 in HeLa cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in HeLa cell lysate.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected TRIM14 in HeLa cell lysate.
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected TRIM14 in human liver tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound TRIM14 peptide with at least one thousand-fold (1,000X) higher than with non-specific control peptide.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected TRIM14 in HeLa cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Target description
Tripartite motif-containing protein 14 (UniProt: Q14142) is encoded by the TRIM14 (also known as KIAA0129) gene (Gene ID: 9830) in human. The protein encoded by TRIM14 is a member of the tripartite motif (TRIM) family. It plays an essential role in the innate immune defense against viruses and bacteria. TRIM14 includes zinc-binding domain (B-box type) and two coiled-coil regions. The B-box zinc finger is responsible for inhibition of SPI1-mediated transcriptional activation. It lacks the N-terminal RING domain, which is found in most other proteins in the TRIM family. TRIM14 expressed in various tissues with highest expression in liver and also in immune-related tissues such as the spleen, thymus, and peripheral blood leukocytes and is undetectable in skeletal muscle. The protein localizes to cytoplasmic bodies; mitochondria and cytoplasmic vesicle (phagosomes). Four alternatively spliced transcript variants for this gene have been described. It is involved in various cellular processes and key functions include Antiviral defense, Autophagy, Inflammatory Response and Cell Proliferation and Survival. TRIM14 is crucial in the host defense against viral infections. Its antiviral activity is significant in the context of infections by viruses such as HIV, influenza, and hepatitis B and C. The antiviral mechanism is by IFN-stimulated genes and Cytokine Signaling in Immune system. TRIM14 is also known to be associated with Spondyloepimetaphyseal Dysplasia of Geneviève Type. TRIM14 may contribute to the pathogenesis of autoimmune diseases if its regulatory mechanisms are disrupted. This ZooMAb® recombinant monoclonal antibody, generated by our proprietary technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Reconstitution
300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
11 - Combustible Solids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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