ZRB2264
Anti-Interleukin-4 Antibody, clone 2C14 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
B-cell stimulatory factor 1 (BSF-1), Binetrakin, Lymphocyte stimulatory factor 1, Pitrakinra
About This Item
biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
2C14, recombinant monoclonal
description
recombinant, expressed in HEK 293 cells
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 17.49 kDa
observed mol wt ~16 kDa
purified by
using Protein A
species reactivity
human
packaging
antibody small pack of 25
greener alternative product characteristics
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enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
technique(s)
ELISA: suitable
affinity binding assay: suitable
immunocytochemistry: suitable
western blot: suitable
isotype
IgGκ
epitope sequence
C-terminal
Protein ID accession no.
UniProt accession no.
greener alternative category
shipped in
ambient
storage temp.
2-8°C
Gene Information
human ... IL4(3565)
Related Categories
General description
Specificity
Immunogen
Application
Evaluated by Western Blotting with human recombinant Interleukin-4.
Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected human recombinant Interleukin-4 .
Tested Applications
Affinity Binding Assay Analysis: The binding affinity of this antibody to Interleukin-4 peptide was at least one thousand-fold (1,000X) higher than that obtained with the non-specific control peptide.
Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Interleukin-4 in MOLT-4 cells with and without treatment with TPA (40 nM, 6 hrs) and Ionomycin (2 uM 6 hrs) and then treated with Brefeldin A (1ug/mL) for the last 5 .
Enzyme Immunoassay (ELISA): A representative lot of this antibody detected Interleukin-4 protein in ELISA application.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Target description
Physical form
Reconstitution
Storage and Stability
Legal Information
Disclaimer
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Storage Class
11 - Combustible Solids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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