ZRB2189
Anti-MRGPRX2 Antibody, clone 1O10 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
Mas-related G-protein coupled receptor member X2
Sign Into View Organizational & Contract Pricing
All Photos(10)
About This Item
UNSPSC Code:
12352203
Recommended Products
biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
1O10, recombinant monoclonal
description
1O10 Clone
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 37.1 kDa
observed mol wt ~40 kDa
purified by
using Protein A
species reactivity
human
species reactivity (predicted by homology)
monkey
packaging
antibody small pack of 25 μL
greener alternative product characteristics
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
technique(s)
affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG
epitope sequence
N-terminal
Protein ID accession no.
UniProt accession no.
greener alternative category
, Aligned
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... MRGPRX2(117194)
Related Categories
General description
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 1O10 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Mas-related G-protein coupled receptor member X2 (MRGPRX2). It targets an epitope within 20 amino acids from the N-terminal, extracellular domain.
Immunogen
KLH-conjugated linear peptide corresponding to 20 amino acids from the N-terminal region of human Mas-related G-protein coupled receptor member X2 (MRGPRX2).
Application
Quality Control Testing
Evaluated by Western Blotting in Human liver tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MRGPRX2 in Human liver tissue lysate.
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected MRGPRX2 in Human dorsal root ganglion tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound MRGPRX2 peptide with a KD of 3.4 x 10-6 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MRGPRX2 in MCF-7 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in Human liver tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected MRGPRX2 in Human liver tissue lysate.
Tested Applications
Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected MRGPRX2 in Human dorsal root ganglion tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound MRGPRX2 peptide with a KD of 3.4 x 10-6 in an affinity binding assay.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MRGPRX2 in MCF-7 cells.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Target description
Mas-related G-protein coupled receptor member X2 (UniProt: Q96LB1; also known as MRGPRX2) is encoded by the MRGPRX2 (also known as MRGX2) gene (Gene ID: 117194) in human. MRGPRX2 is a multi-pass membrane protein with four extracellular domains, seven transmembrane domains, and four cytosolic domains. It is mainly expressed in mast cells; however, it has limited expression in peripheral and central nervous system, higher levels reported in small sensory cells in the dorsal root ganglion. It serves as a mast cell-specific receptor for basic secretagogues drug and for endogenous and exogenous peptides. Cortistain that has role in sleep regulation, locomotor activity, and cortical function has been recognized a high potency ligand for MRGPRX2. MRGPRX2 has also been linked to pseudo-allergic drug reactions to certain drugs, such as ciprofloxacin, and atracurium. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Navinés-Ferrer, A., et al. (2018). Sci Rep. 8; 11628; McNeil, BD., et al. (2015). Nature. 519(7542); 237-241; Robas, N., et al. (2003). J. Biol. Chem. 278(45); 44400-44404).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Reconstitution
300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service