ZRB2131
Anti-BMAL1/ARNTL Antibody, clone 1J12 ZooMAb® Rabbit Monoclonal
recombinant, expressed in HEK 293 cells
Synonym(s):
Aryl hydrocarbon receptor nuclear translocator-like protein 1;Basic-helix-loop-helix-PAS protein MOP3;Brain and muscle ARNT-like 1;Class E basic helix-loop-helix protein 5;bHLHe5, Member of PAS protein 3;PAS domain-containing protein 3;bHLH-PAS protein JA
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
recombinant
expressed in HEK 293 cells
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
1J12, recombinant monoclonal
description
1J12 Clone
product line
ZooMAb® learn more
form
lyophilized
mol wt
calculated mol wt 68.76 kDa
observed mol wt ~70 kDa
purified by
using Protein A
species reactivity
mouse, human
packaging
antibody small pack of 25 μL
enhanced validation
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
technique(s)
ELISA: suitable
affinity binding assay: suitable
western blot: suitable
isotype
IgG
epitope sequence
C-terminal
Protein ID accession no.
UniProt accession no.
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... ARNTL(406)
General description
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.
Specificity
Clone 1J12 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects BMAL1/ARNTL. It targets an epitope within 19 amino acids from the C-terminal region.
Immunogen
KLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal region of human BMAL1/ARNTL.
Application
Quality Control Testing
Evaluated by Western Blotting in NIH3T3 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected BMAL1/ARNTL in NIH3T3 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected recombinant human BMAL1/ARNTL
Enzyme Immunoassay (ELISA) Analysis: Various dilutions from a representative lot detected ABP and His tagged recombinant human BMAL1/ARNTL
Affinity Binding Assay: A representative lot of this antibody bound BMAL1/ARNTL peptide with a KD of 5.8 x 10-6 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Evaluated by Western Blotting in NIH3T3 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected BMAL1/ARNTL in NIH3T3 cell lysate.
Tested Applications
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected recombinant human BMAL1/ARNTL
Enzyme Immunoassay (ELISA) Analysis: Various dilutions from a representative lot detected ABP and His tagged recombinant human BMAL1/ARNTL
Affinity Binding Assay: A representative lot of this antibody bound BMAL1/ARNTL peptide with a KD of 5.8 x 10-6 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.
Target description
Aryl hydrocarbon receptor nuclear translocator-like protein 1 (UniProt: O00327; also known as Basic-helix-loop-helix-PAS protein MOP3, Brain and muscle ARNT-like 1, Class E basic helix-loop-helix protein 5, bHLHe5, Member of PAS protein 3, PAS domain-containing protein 3, bHLH-PAS protein JAP3) is encoded by the ARNTL (also known as BHLHE5, BMAL1, MOP3, PASD3) gene (Gene ID: 406) in human. BMAL1 is a transcriptional activator that forms a core component of the circadian clock. These components include ARNTL/BMAL1, CLOCK, NPAS2, ARNTL2/BMAL2, PER1, PER2, PER3, CRY1, and CRY2. CLOCK or NPAS2 and ARNTL/BMAL1 or ARNTL2/BMAL2 form the positive limb of the feedback loop. They act in the form of a heterodimer and activate the transcription of core clock genes and clock-controlled genes that are involved in key metabolic processes. PER1/2/3 and CRY1/2, act as transcriptional repressors and form the negative limb of the feedback loop. They interact with the CLOCK|NPAS2-ARNTL/BMAL1|ARNTL2/BMAL2 heterodimer inhibiting its activity. BMLA1 is reported to be phosphorylated upon dimerization with CLOCK and this phosphorylation enhances its transcriptional activity and reduces the stability of the CLOCK-ARNTL/BMAL1 heterodimer by promoting its degradation. BMAL1 is shown to positively regulate myogenesis and negatively regulate adipogenesis via the transcriptional control of the genes of the canonical Wnt signaling pathway. BMAL1 is the only component of the mammalian circadian clock whose deletion in a murine model is shown to generate arrhythmicity. BMAL1 null-mice are reported to have reproductive problems, reduced locomotor activity, and premature aging. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Wang, Z., et al. (2013). Cell Res. 23(2); 213-224).
Physical form
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.
Reconstitution
0.3 mg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
Storage and Stability
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
Legal Information
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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