ZRB1957

Anti-KEAP1 Antibody, clone 3B9 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Cytosolic inhibitor of Nrf2, INrf2, Kelch-like ECH-associated protein 1, Kelch-like protein 19
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 3B9, recombinant monoclonal
• description: 3B9 Clone
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 69.66 kDa
• purified by: using Protein A
• species reactivity: human, rat, mouse
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• technique(s): ELISA: suitable; affinity binding assay: suitable; immunocytochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal half
• Protein ID accession no.: NP_036421
• UniProt accession no.: Q14145
• greener alternative category: Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• Gene Information: human ... KEAP1(9817)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry. This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies. Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 3B9 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects KEAP1. It targets an epitope within 17 amino acids from the Kelch repeat 2 within the C-terminal half.

Immunogen

KLH-conjugated linear peptide corresponding to 17 amino acids from the Kelch repeat 2 region within the C-terminal half of human Kelch-like ECH-associated protein 1 (KEAP1).

Application

Quality Control Testing

Evaluated by Western Blotting in C6 cell lysate.

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detects KEAP1 in C6 cell lysate.

Tested Applications

Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected KEAP1 in lysates from HeLa, MCF-7, NIH3T3 cells, and Mouse Kidney tissue and 1:10,000 dilution detected full-length recombinant Human KEAP1.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected KEAP1 in U2OS cells.

Affinity Binding Assay: A representative lot of this antibody bound KEAP1 peptide with a KD of 5.4 x 10-7 in an affinity binding assay.

Enzyme Immunoassay (ELISA) Analysis: A serial of dilutions from a representative lot detected His-GST-tagged, full-length, recombinant Human KEAP1.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Kelch-like ECH-associated protein 1 (UniProt: Q14145;  also known as Cytosolic inhibitor of Nrf2, INrf2, Kelch-like protein 19) is encoded by the KEAP1 (also known as INRF2, KIAA0132, KLHL10) gene (Gene ID: 9817) in human. KEAP1 is broadly expressed protein with highest levels observed in skeletal muscle. It serves as a substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex that regulates the response to oxidative stress by targeting Nrf2 for ubiquitination and degradation. It is reported to be a key regulator of the Nrf2, which transactivates the Antioxidant Response Element (ARE) and upregulates numerous proteins involved in antioxidant defense. Under basal conditions, KEAP1 targets Nrf2 for ubiquitination and proteolytic degradation.  However, in response to oxidative stress, different electrophile metabolites trigger non-enzymatic covalent modifications of highly reactive cysteine residues in KEAP1, leading to inactivate the ubiquitin ligase activity of the BCR(KEAP1) complex. This leads to nuclear accumulation of Nrf2 and expression of phase II detoxifying enzymes. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Buckley, BJ., et al. (2008). Free Radic. Biol. Med. 44(4); 692-698; Gao, L., et al. (2007). J. Biol. Chem. 282(4); 2529-2537; Furukawa, M., and Xiong, Y. (2005). Mol. Cell Biol. 25(1); 162-171).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 µL.

Reconstitution

300 µg/mL after reconstitution at 25 µL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 µL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable