ZRB1750

Anti-LRBA Antibody, clone 3H22 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Lipopolysaccharide-responsive and beige-like anchor protein; Beige-like protein;CDC4-like protein
• UNSPSC Code: 12352203
• NACRES: NA.43

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 3H22, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 319.11 kDa; observed mol wt ~320 kDa
• purified by: using Protein A
• species reactivity: mouse, human
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): flow cytometry: suitable; immunocytochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: N-terminal half
• Protein ID accession no.: NP_006717
• UniProt accession no.: P50851
• greener alternative category: Aligned,
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... LRBA(987)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 3H22 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Lipopolysaccharide-responsive and beige-like anchor protein (LRBA). It targets and epitope within the N-terminal half.

Immunogen

His-tagged recombinant fragment corresponding to 244 amino acids from the N-terminal half of human Lipopolysaccharide-responsive and beige-like anchor protein (LRBA).

Application

Quality Control Testing

Evaluated by Western Blotting in U2OS cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected LRBA in U2OS cell lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected LRBA in NIH3T3 cell lysate.

Flow Cytometry Analysis: 0.1 μg from a representative lot detected LRBA in one million U2OS cells.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected LRBA in NIH3T3 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Evaluated by Western Blotting in U2OS cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected LRBA in U2OS cell lysate.

Target description

Lipopolysaccharide-responsive and beige-like anchor protein (UniProt: P50851; also known as Beige-like protein, CDC4-like protein) is encoded by the LRBA (also known as BGL, CDC4L, LBA) gene (Gene ID: 987) in human. LRBA is a single-pass membrane protein that displays ubiquitous expression in normal tissues, but its expression is elevated in malignancy. In immune cells. It is found in all membrane compartments that are associated with receptor signaling, including the plasma membrane, Golgi complex, clathrin-coated pits, and trans-endocytic vesicles. Its expression is induced in B cells and macrophages by bacterial lipopolysaccharides (LPS). Two isoforms of LRBA have been reported that are produced by alternative splicing. LRBA contains 2 BEACH (Beige and Chediak-Higashi) domains and six WD repeats at its C-terminal. LRBA may be involved in coupling signal transduction and vesicle trafficking to enable polarized secretion and/or membrane deposition of immune effector molecules. It coordinates signaling of immune receptors to promote effector function and plays a crucial role in immune regulation. Mutations in LRBA gene lead to disorders of the immune system, including defective B-cell differentiation and decreased or absent antibody production, which results in early-childhood onset of recurrent infections. Mutations in LRBA gene are associated with defective B-cell differentiation and decreased or absent antibody production. This ZooMAbZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Park, MY et al. (2016). Nat. Sci. Rep. 6; 36568; Wang, J-W., et al. (2014). J. Allergy Clin. Immunol. 133(2; suppl); AB251).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable