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ZRB1440

Sigma-Aldrich

Anti-MRC1 Antibody, clone 1C9 ZooMAb® Rabbit Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

Synonym(s):
C-type lectin domain family 13 member D, C-type lectin domain family 13 member D-like, CD206, Human mannose receptor, MMR, Macrophage mannose receptor 1, Macrophage mannose receptor 1-like protein 1, hMR

biological source

rabbit (recombinant)

recombinant

expressed in HEK 293 cells

conjugate

unconjugated

antibody form

purified antibody

antibody product type

primary antibodies

clone

1C9, monoclonal
recombinant monoclonal

product line

ZooMAb® learn more

form

lyophilized

mol wt

calculated mol wt 166.01 kDa
observed mol wt 180 kDa

species reactivity

mouse, human

species reactivity (predicted by homology)

monkey

packaging

antibody small pack of 25 μL

greener alternative product characteristics

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
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enhanced validation

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

affinity binding assay: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

isotype

IgG

UniProt accession no.

greener alternative category

shipped in

ambient

storage temp.

2-8°C

Gene Information

human ... MRC1(4360)

General description

ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone 1C9 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Macrophage mannose receptor 1 (MRC1). It targets an epitope within 25 amino acids from the C-terminal region.

Immunogen

KLH-conjugated linear peptide corresponding to 25 amino acids from the C-terminal region of human Macrophage mannose receptor 1 (MRC1).

Application

Anti-MRC1, clone 1C9 ZooMAb, Cat. No. ZRB1440, is a recombinant Rabbit monoclonal antibody that specifically targets MRC1 and is tested in Affinity Binding Assay, Flow Cytometry, Immunocytochemistry, Immunohistochemistry, and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected MRC1 in Human lymph node tissue lysate.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected MRC1 in human lung tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected MRC1 in RAW 264.7 cells.Flow Cytometry Analysis: 0.01 μg from a representative lot detected MRC1 in RAW 264.7 cells.

Affinity Binding Assay: A representative lot of this antibody bound MRC1 peptide with a KD of 4.6 x 10-8 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Macrophage mannose receptor 1 (UniProt: P22897; also known as MMR, C-type lectin domain family 13 member D, C-type lectin domain family 13 member D-like, Human mannose receptor, hMR, Macrophage mannose receptor 1-like protein 1, CD206, MRC1) is encoded by the MRC1 (also known as CLEC13D, CLEC13DL, MRC1L1) gene (Gene ID: 4360) in human. MRC1 is a single-pass type I membrane protein that mediates the endocytosis of glycoproteins by macrophages. It also functions in phagocytosis and plays a role in immune homeostasis by scavenging unwanted mannoglycoproteins. It is expressed on the surface of macrophages, immature dendritic cells and liver sinusoidal endothelial cells. It is a C-type lectin with eight C-type lectin domains and can bind both sulfated and non-sulfated polysaccharide chains. MRC1 is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to generate the mature form that contains a large extracellular domain (aa 19-1389), a transmembrane domain (aa 1390-13410), and a cytoplasmic tail (aa 1411-1456). The extracellular region includes an N-terminal cysteine-rich domain that binds glycoproteins bearing sulfated sugars that terminate in 4-SO4GalNAc, a fibronectin II (FNII) domain, and eight carbohydrate recognition domains that bind sugars like mannose and fucose with high affinity. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in lyophilized from PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

0.03 mg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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