ZRB1395

Anti-TET2 Antibody, clone 4L14 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 4L14, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 223.81 kDa; observed mol wt ~250 kDa
• species reactivity: mouse, human, rat
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): affinity binding assay: suitable; immunocytochemistry: suitable; western blot: suitable
• isotype: IgG
• epitope sequence: N-terminus
• Protein ID accession no.: NP_001120680
• UniProt accession no.: Q6N021
• greener alternative category: Aligned,
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

Specificity

Clone 4L14 is a ZooMAb^® rabbit recombinant monoclonal antibody that specifically detects Methylcytosine dioxygenase TET2. It targets an epitope within the N-terminal region.

Immunogen

His-tagged recombinant fragment corresponding to the first 300 amino acids from the N-terminal region of human Methylcytosine dioxygenase TET2.

Application

Quality Control Testing

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: A 1:10,000 dilution of this antibody detected TET2 in HeLa cell lysate.

Tested applications

Western Blotting Analysis: A 1:10,000 dilution from a representative lot detected TET2 in TF-1, L6, and NIH3T3 cell lysates.

Affinity Binding Assay: A representative lot of this antibody bound recombinant TET2 fragment with a KD of 4.1 x 10-7 in an affinity binding assay.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected TET2 in HeLa, HUVEC, and NIH3T3 cells.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-TET2, clone 4L14 ZooMAb^®, Cat. No. ZRB1395, is a recombinant Rabbit monoclonal antibody that detects TET2 and is tested for use in Affinity Binding Assay, Immunocytochemistry, and Western Blotting.

Target description

Methylcytosine dioxygenase TET2 (UniProt: Q6N021; also known as EC:1.14.11.n2, TET2) is encoded by the TET2 (also known as KIAA1546, Nbla00191) gene (Gene ID: 54790) in human. TET2 is an evolutionarily conserved nuclear protein with several Fe2+ and Zn2+ binding sites. It is highly expressed in hematopoietic stem/progenitor cells and serves as a dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC) and plays a key role in active DNA demethylation. It displays a preference for 5-hydroxymethylcytosine in CpG motifs. TET2 also mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC), and conversion of 5fC to 5-carboxylcytosine (5caC). Methylation at the C5 position of cytosine bases is an epigenetic modification of the mammalian genome which plays an important role in transcriptional regulation. In addition to its role in DNA demethylation, it is also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes that helps in promoting histone H2B GlcNAcylation by OGT. TET2 is reported to be frequently mutated in myeloproliferative disorders that involve cellular proliferation of one or more hematologic cell lines in the peripheral blood, distinct from acute leukemia. These disorders include essential thrombocythemia, polycythemia vera, and primary myelofibrosis. Deletion of TET2 in primary bone marrow cells is shown to increase the percentage of immature c-Kit+ Lin cells, indicating that loss of TET2 can affect stem/progenitor cell differentiation. Three isoforms of TET2 have been described that are produced by alternative splicing. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Moran-Crusio, K., et al. (2011). Cancer Cell. 20(1); 11-24; Feng, Y., et al. (2019). Front. Oncol. 9; 10).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1