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Y1771

Sigma-Aldrich

Yeast Synthetic Drop-out Medium Supplements

without uracil, leucine, and tryptophan

Synonym(s):

Synthetic yeast drop-out mix, Yeast Synthetic Drop-out Media Supplements

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About This Item

MDL number:
MFCD01095679
UNSPSC Code:
41106212
NACRES:
NA.85

Quality Level

200

form

powder

technique(s)

transformation: suitable

application(s)

microbiology

storage temp.

room temp

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Application

Yeast Synthetic Drop-out Medium Supplements, without uracil, leucine, and tryptophan has been used for screening:

  • the transformants of the PO1f strains which carried the pCRISPRyl_URA3sgRNA plasmids
  • Yarrowia lipolytica PO1f strain positive transformants
  • CRISPR- and RNA-assisted in vivo directed evolution (CRAIDE) mutants
The selection of plasmids in yeast is based on the use of auxotrophic mutant strains that cannot grow without a specific media component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Sigma′s Yeast Synthetic Drop-Out Media Supplements create a richer medium for better yield and growth rate, and increase the probability of successful transformations when screening libraries or performing gene knock-outs.

Other Notes

Mixtures of amino acids and other nutrients to be added to Yeast Nitrogen Base Without Amino Acids.

Preparation Note

Dissolve 1.46 g of supplement per liter of medium.

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pictograms

Corrosion
GHS05

signalword

Danger

hcodes

H318

Hazard Classifications

Eye Dam. 1

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Paul Swietalski et al.
Microbial cell factories, 19(1), 100-100 (2020-05-13)
The secretory production of recombinant proteins in yeast simplifies isolation and purification but also faces possible complications due to the complexity of the secretory pathway. Therefore, correct folding, maturation and intracellular transport of the recombinant proteins are important processing steps

Articles

Introduction to Yeast Media

Technical Article on yeast media. Yeasts are eukaryotic microorganisms whose genomes have been comprehensively studied and some have been sequenced.

Protocols

Yeast Drop Out

Yeast Drop Out Bulletin. The selection of plasmids in yeast is based on the use of auxotrophic mutant strains, which cannot grow without a specific medium component (an amino acid, purine or pyrimidine). Transformation with a plasmid containing the mutated gene enables the transformant to grow on a medium lacking the required component. Although yeast can grow on a synthetic medium without any amino acids, better yield and growth rate can be achieved on richer media.

Yeast Growth Protocols

Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells. Yeast cultures can be grown, maintained, and stored in liquid media or on agar plates using techniques similar to those for bacterial cultures.

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