U211000
DMT-2′O-TBDMS-rU Phosphoramidite
Synonym(s):
5′-O-[bis(4-methoxyphenyl)phenylmethyl]-2′-O-[(1,1-dimethylethyl)dimethylsilyl]-uridine, 3′-[2-cyanoethyl N,N-bis(1-methylethyl)phosphoramidite], DMT-2′-O-TBDMS-rU amidite
About This Item
Recommended Products
biological source
non-animal source (no BSE/TSE risk)
Quality Level
product line
Proligo Reagents
assay
≥99% (31P-NMR)
≥99.0% (reversed phase HPLC)
form
powder
impurities
≤0.3 wt. % water content (Karl Fischer)
≤0.5% P(III) Impurities 100-169ppm (31P-NMR)
≤0.5% single Impurity (reversed phase HPLC)
≤3 wt. % residual Solvent content
color
white to off-white
λ
conforms (UV/VIS Identity)
suitability
conforms to structure for H-NMR
conforms to structure for LC-MS
nucleoside profile
base: uridine
base protecting group: none
2' protecting group: TBDMS
5' protecting group: DMT
deprotection: fast/standard
storage temp.
−20°C
SMILES string
COc1ccc(cc1)C(OC[C@H]2O[C@H]([C@H](O[Si](C)(C)C(C)(C)C)[C@@H]2OP(OCCC#N)N(C(C)C)C(C)C)N3C=CC(=O)NC3=O)(c4ccccc4)c5ccc(OC)cc5
InChI
1S/C45H61N4O9PSi/c1-31(2)49(32(3)4)59(55-29-15-27-46)57-40-38(56-42(48-28-26-39(50)47-43(48)51)41(40)58-60(10,11)44(5,6)7)30-54-45(33-16-13-12-14-17-33,34-18-22-36(52-8)23-19-34)35-20-24-37(53-9)25-21-35/h12-14,16-26,28,31-32,38,40-42H,15,29-30H2,1-11H3,(H,47,50,51)/t38-,40-,41-,42-,59?/m1/s1
InChI key
SKNLXHRBXYGJOC-ZMHKPELYSA-N
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General description
Application
Features and Benefits
- Industry-standard 2′O-TBDMS protective group
- Consistent lot-to-lot purity and performance
- Compatible with deprotection methods based on methylamine or AMA
- Standard RNA phosphoramidites provide excellent coupling results when used with ETT or BTT as activator; best results are obtained with Activator 42
- Capping with standard acetic anhydride capping reagent rather than with Fast Deprotection Cap A
- Manufactured under a certified ISO 9001 quality system
Other Notes
- Catalytic agents (ribozymes)
- Affinity ligands (aptamers)
- Agents to induce gene silencing (RNA interference)
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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