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T1952

Sigma-Aldrich

Trichostatin A, Ready Made Solution

5 mM in DMSO (0.2 μm-filtered), from Streptomyces sp.

Synonym(s):
[R-(E,E)]-7-[4-(Dimethylamino)phenyl]-N-hydroxy-4,6-dimethyl-7-oxo-2,4-heptadienamide, TSA
Empirical Formula (Hill Notation):
C17H22N2O3
CAS Number:
Molecular Weight:
302.37
NACRES:
NA.32

Quality Level

biological source

Streptomyces sp.

assay

≥98% (HPLC)

form

DMSO solution

concentration

5 mM in DMSO (0.2 μm-filtered)

technique(s)

cell culture | mammalian: suitable

antibiotic activity spectrum

fungi
neoplastics

Mode of action

enzyme | inhibits

shipped in

dry ice

storage temp.

−20°C

InChI

1S/C17H22N2O3/c1-12(5-10-16(20)18-22)11-13(2)17(21)14-6-8-15(9-7-14)19(3)4/h5-11,13,22H,1-4H3,(H,18,20)/b10-5+,12-11+

InChI key

RTKIYFITIVXBLE-WKWSCTOISA-N

General description

Trichostatin A is a compound of primary hydroxamic acid.

Application

Trichostatin A, ready-made solution has been used:
  • as a histone deacetylase inhibitor to study its effect on transcriptome changes by stem cell testing
  • to inhibit histone deacetylase (HDAC) class I, II, or III in primary pituitary cell cultures and to investigate insulin control of endogenous human growth hormone gene (hGH)
  • to treat cells for the HDAC inhibition experiments

Biochem/physiol Actions

Trichostatin A (TSA) is a Streptomyces metabolite, which specifically inhibits mammalian histone deacetylase at a nanomolar concentration and causes accumulation of highly acetylated histone molecules in mammalian cells. For that reason, trichostatin A is a tool to study the consequences of histone acetylation in vivo. Trichostatin A induces cell differentiation, cell cycle arrest, reversal of transformed cells morphology, and apoptosis and is able to modulate transcription. TSA has been used to establish a new cloning technique, which increases the success rates for mouse cloning.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

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Certificate of Origin

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