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Key Documents

T0831

Sigma-Aldrich

Tissue plasminogen activator human

Synonym(s):

tPA

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About This Item

Enzyme Commission number:
MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

form

lyophilized powder

Quality Level

specific activity

≥300 KU/mg protein

mol wt

~70 kDa

impurities

HIV, HCV, and HBsAg, tested negative

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... PLAT(5327)

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General description

Tissue plasminogen activator (tPA) is a protease of the S1 family (trypsin family) and is found in a wide variety of mammalian tissues, especially endothelial cells. tPA is secreted as a single chain precursor, which is cleaved to a two-chain form by plasmin. Supplied in the single chain form with a molecular weight of ~70 kDa. The Tissue Plasminogen Activator (tPA) gene is mapped to human chromosome 8p11.21 and encodes a protease of 69 kDa majorly containing serine amino acid residue. It is predominantly expressed in endothelial cells.

Application

Tissue plasminogen activator has been used in a study to assess treatment risks of intracranial hemorrhage among patients with acute ischemic stroke. Tissue plasminogen activator has also been used in a study to investigate the successful treatment of early thrombosis of HeartWare left ventricular assist devices with intraventricular thrombolytics. Human tissue plasminogen activator has been used to study the effect of tyrosinase-related protein 2 (TRP-2) expression on melanin production in sheep.

Biochem/physiol Actions

tPA is a potent activator of fibrinolysis and converts plasminogen into plasmin and also initiates cell signaling. The plasminogen activation system may also be associated with degradation of amyloid fibrils. It is also known to mediate inflammatory and extracellular matrix remodeling processes and is predicted to participate in disease progression of intracranial aneurysm indicating brain vascular malformations. Upregulation of the gene is observed in aortic aneurysms.

Physical form

Lyophilized powder, from 100 mM PBS (pH 7.4), 3.5 mg/mL L-arginine, and 0.001% Tween® 80

Preparation Note

Reconstitute with dH2O to a final concentration of 1 mg/mL (e.g. 100 μg should be reconstituted in 100 μL).

Legal Information

TWEEN is a registered trademark of Croda International PLC

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Warning

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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Converting Tissue Type Plasminogen Activator into a Zymogen IMPORTANT ROLE OF Lys156.
Tachias K and Madison E L
The Journal of Biological Chemistry, 272(1), 28-31 (1997)
Design, synthesis, and characterization of urokinase plasminogen-activator-sensitive near-infrared reporter.
Law B, et al.
Chemistry & Biology, 11(1), 99-106 (2004)
Successful treatment of early thrombosis of HeartWare left ventricular assist device with intraventricular thrombolytics.
Kamouh A, et al.
The Annals of Thoracic Surgery, 94(1), 281-283 (2012)
Differential expression assay of chromosome arm 8p genes identifies Frizzled-related (FRP1/FRZB) and Fibroblast Growth Factor Receptor 1 (FGFR1) as candidate breast cancer genes.
Ugolini F, et al.
Oncogene, 18(10), 1903-1903 (1999)
Amorphous protein aggregates stimulate plasminogen activation, leading to release of cytotoxic fragments that are clients for extracellular chaperones.
Constantinescu P, et al.
The Journal of Biological Chemistry (2017)

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