T0825

Anti-Transportin 1 antibody, Mouse monoclonal

clone D45, purified from hybridoma cell culture

• Synonym(s): Anti-IPO2, Anti-KPNB2, Anti-MIP, Anti-MIP1, Anti-TRN
• MDL number: MFCD06202150
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: mouse
• conjugate: unconjugated
• antibody form: purified from hybridoma cell culture
• antibody product type: primary antibodies
• clone: D45, monoclonal
• form: buffered aqueous solution
• mol wt: antigen ~90 kDa
• species reactivity: mouse, canine, rat, human, bovine
• technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; microarray: suitable; western blot: 0.1-0.2 μg/mL using HeLa nuclear extract
• isotype: IgG1
• UniProt accession no.: Q92973
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... TNPO1(3842) ; mouse ... Tnpo1(238799) ; rat ... Tnpo1(309126)

Description

General description

Transportin 1 (TNPO1 or TRN1) also known as karyopherin-β2, is a nuclear import protein and belongs to importin-β family. In the human chromosome the TNPO1 gene is localized on 5q13.2.(10)

Immunogen

recombinant human transportin 1.

Application

Monoclonal Anti-Transportin 1 antibody produced in mouse has been used in western blotting, immunofluorescence, and immunostaining.

Biochem/physiol Actions

Transportin 1 (TNPO1 or TRN1) recognizes the nuclear localization signals (NLS) and binds to the nuclear pore complex (NPC) to facilitate the transport of substances from cytoplasm to nucleus. TRN1 transports viral, and ribosomal proteins with the aid of RanGTP (Ras associated nuclear protein-GTP). TRN1 is also known to regulate the key events in the cell cycle assembly like nuclear membrane and nuclear pore. Mutations in TRN1 might cause amyotrophic lateral sclerosis due to mislocalisation of FUS (fused in sarcoma) protein. TRN1 is associated with regulation of circadian rhythm through nuclear localization of PER1 (period circadian protein homolog 1) protein.

Target description

Transportin 1 encodes the beta subunit of the karyopherin receptor complex which interacts with nuclear localization signals to target nuclear proteins to the nucleus. The karyopherin receptor complex is a heterodimer of an alpha subunit which recognizes

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable