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SRP6546

Sigma-Aldrich

Procathepsin L human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

Synonym(s):

CTSL, CTSL1

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

assay

≥90% (SDS-PAGE)

form

liquid

mol wt

39.4 kDa (18-333 aa + N-terminal poly-his tag.)

packaging

pkg of 1 mg
pkg of 100 μg

concentration

1 mg/mL

UniProt accession no.

shipped in

wet ice

storage temp.

−70°C

Gene Information

human ... CTSL1(1514)

General description

Cathepsin L is a lysosomal protease that can be secreted from the cell and is implicated to play a role in protein degradation, arthritis, apoptosis, and cancer. TheProcathepsin L can be auto-catalytically activated to mature Cathepsin L (~24 kDa) by adding an equal volume of 0.2 M NaOAc, 5 mM EDTA pH 4.0 containing fresh 5 mM DTT at RT. The enzyme is fully activated within 30 min of incubation at RT. After activation mature Cathepsin L is highly auto-proteolytic at pH 4.0, and care must be taken to avoid self-proteolysis. If the activated enzyme is not used immediately, methylmethanthiosulfonate (1mM final concentration; MeS-SO2Me; MMTS) could be added and sample should be frozen at -80°C. The activity can be then restored by adding L-cysteine (3 M excess over MMTS) to the enzyme solution.

Physical form

1 mg/mL in 25 mM Na2HPO4, 500 mM NaCl, pH 7.0.

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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