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SRP5099

Sigma-Aldrich

WNK1 (181-507), active, GST tagged human

PRECISIO® Kinase, recombinant, expressed in baculovirus infected Sf9 cells, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

Synonym(s):

HSAN2, HSN2, KDP, KIAA0344, MGC163339, MGC163341, PRKWNK1, PSK, p65

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

recombinant

expressed in baculovirus infected Sf9 cells

product line

PRECISIO® Kinase

assay

≥70% (SDS-PAGE)

form

buffered aqueous glycerol solution

specific activity

25-33 nmol/min·mg

mol wt

~67 kDa

NCBI accession no.

NM_018979

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... WNK1(65125)

General description

WNK1 is a member of the WNK subfamily of serine/threonine protein kinases that is a key regulator of blood pressure by controlling the transport of sodium and chloride ions. Mutations in WNK1 have been associated with pseudohypoaldosteronism type II and hereditary sensory neuropathy type II. WNK1 is a regulator of blood pressure and deficiency of this protein in mice lowers the blood pressure. WNK1 can regulate the Ca(2+) sensing and the subsequent Ca(2+)-dependent interactions mediated by synaptotagmin C2 domains and WNK1 exhibits additive CFTR inhibition.

Physical form

Supplied in 50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

Preparation Note

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

Legal Information

PRECISIO is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Byung-Hoon Lee et al.
Molecular cell, 15(5), 741-751 (2004-09-08)
WNK (with no lysine [K]) protein kinases were named for their unique active site organization. Mutations in WNK1 and WNK4 cause a familial form of hypertension by undefined mechanisms. Here, we report that WNK1 selectively binds to and phosphorylates synaptotagmin
Brian P Zambrowicz et al.
Proceedings of the National Academy of Sciences of the United States of America, 100(24), 14109-14114 (2003-11-12)
The availability of both the mouse and human genome sequences allows for the systematic discovery of human gene function through the use of the mouse as a model system. To accelerate the genetic determination of gene function, we have developed

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