All Photos(1)

SRP4908

Sigma-Aldrich

Visfatin

recombinant, expressed in E. coli, ≥95% (SDS-PAGE), ≥95% (HPLC)

Synonym(s):
Nicotinamide phosphoribosyltransferase, Pre-B-cell colony-enhancing factor 1 homolog

biological source

mouse

recombinant

expressed in E. coli

assay

≥95% (HPLC)
≥95% (SDS-PAGE)

form

lyophilized

mol wt

~55.0 kDa

packaging

pkg of 10 μg

storage condition

avoid repeated freeze/thaw cycles

impurities

endotoxin, tested

NCBI accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

mouse ... Nampt(59027)

General description

Visfatin is also called pre-B cell colony enhancing factor, nicotinamide phosphoribosyltransferase, pre-B-cell colony-enhancing factor 1 homolog, NAmPRTase, Nampt and PBEF. Visfatin is an adipocytokine produced by visceral fat.

Biochem/physiol Actions

Visfatin has a role in mammalian nicotinamide adenine dinucleotide (NAD+) biosynthesis. Studies have shown that mice deficient in visfatin in the forebrain excitatory neurons show hyperactivity, diminished anxiety-like behaviors and impaired learning and memory. It has also been shown that visfatin stimulates glucose uptake and adenosine monophosphate-activated protein kinase (AMPK) phosphorylation in mouse C2C12 skeletal muscle cells.

Physical form

Lyophilized without any additives.

Reconstitution

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.
Reconstitute in PBS to the concentration of 0.1-1 mg/mL.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

The evolutionary portrait of metazoan NAD salvage.
Carneiro J
PLoS ONE, 8(5), e64674-e64674 (2013)
Visfatin, a novel adipokine, stimulates glucose uptake through the Ca2 +-dependent AMPK-p38 MAPK pathway in C2C12 skeletal muscle cells.
Lee JO
Journal of Molecular Endocrinology, 54(3), 251-262 (2015)
Liana Roberts Stein et al.
Brain research bulletin, 119(Pt A), 41-51 (2015-10-21)
Nicotinamide adenine dinucleotide (NAD(+)) is an essential coenzyme/cosubstrate for many biological processes in cellular metabolism. The rate-limiting step in the major pathway of mammalian NAD(+) biosynthesis is mediated by nicotinamide phosphoribosyltransferase (Nampt). Previously, we showed that mice lacking Nampt in
Yuri L Boteon et al.
PloS one, 13(7), e0201419-e0201419 (2018-07-26)
Pharmacological defatting of rat hepatocytes and hepatoma cell lines suggests that the same method could be used to ameliorate macrovesicular steatosis in moderate to severely fatty livers. However there is no data assessing the effects of those drugs on primary
Xiaoqun Lv et al.
Journal of Cancer, 6(9), 849-858 (2015-08-19)
Colorectal cancer (CRC) is the third most common cancer disease. Here we examined Nampt expression in patients with CRC and the effect of Nampt on cell viability in CRC cells. Nampt protein was overexpressed in colorectal adenoma as well as

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